Detailed information of TA system TA202634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2470991..2471629
• Replicon: chromosome
• Accession: NZ_CP074680
• Organism: Escherichia coli strain MEI008

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KIM92_RS11950
• Protein ID: WP_000813794.1
• Coordinates: 2471453..2471629 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KIM92_RS11945
• Protein ID: WP_001270286.1
• Coordinates: 2470991..2471407 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM92_RS11920 (2466308)	2466308..2466961	-	654	Protein_2331	ABC transporter ATP-binding protein	-
KIM92_RS11930 (2467730)	2467730..2468098	-	369	Protein_2333	ABC transporter ATP-binding protein	-
KIM92_RS11935 (2468116)	2468116..2469261	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
KIM92_RS11940 (2469506)	2469506..2470912	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
KIM92_RS11945 (2470991)	2470991..2471407	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KIM92_RS11950 (2471453)	2471453..2471629	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KIM92_RS11955 (2471851)	2471851..2472081	+	231	WP_000494244.1	YncJ family protein	-
KIM92_RS11960 (2472173)	2472173..2474134	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KIM92_RS11965 (2474207)	2474207..2474743	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
KIM92_RS11970 (2474835)	2474835..2476010	+	1176	WP_001236319.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2476050..2477315	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T202634 WP_000813794.1 NZ_CP074680:c2471629-2471453 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T202634 NZ_CP099535:2691274-2691489 [Pantoea ananatis]
ATGAACAAAACCCTAACTAAAACTGATTATTTGATGCGACTGAGGCGCTGTCGTTCTATTGACACGCTGGAACGCGTGAT
TGAAAAAAATAAGTACGAATTATCCGATGAAGAATTAGCAGTATTTTACTCAGCAGCCGATCACCGCCTGGCTGAACTGA
CGATGAACAAGCTCTACGACAAAGTCCCGGTGTCAGTCTGGAAATTTGTACGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT202634 WP_001270286.1 NZ_CP074680:c2471407-2470991 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT202634 NZ_CP099535:2690872-2691249 [Pantoea ananatis]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACTTGTGTGAGAGTCTGTTTGACGAAAGCATGGC
GACATTAACTGACAGTCATCACGGCTGGGTTAATGATCCCACTGCCGAAAGCAATTTACAGCTAAACGATTTGATTGAGC
ACATTGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGACGAAGCACTGATTACCCAAATCGATGAATATCTT
GATGATACCTTTATGCTGTTTAGCAACTACAGTGTAAATACTCAGGATCTTCAGCGCTGGCAACGCTCAGCGAAACGTTT
ATTTAATGTGTTTACTGAAGAGTGCGCTTTTCTTCAACAGCCAAGCCATTCATTATAG

Structures

Toxin

• PDB: 6HPB