Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2470991..2471629 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KIM92_RS11950 Protein ID WP_000813794.1
Coordinates 2471453..2471629 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KIM92_RS11945 Protein ID WP_001270286.1
Coordinates 2470991..2471407 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS11920 (2466308) 2466308..2466961 - 654 Protein_2331 ABC transporter ATP-binding protein -
KIM92_RS11930 (2467730) 2467730..2468098 - 369 Protein_2333 ABC transporter ATP-binding protein -
KIM92_RS11935 (2468116) 2468116..2469261 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
KIM92_RS11940 (2469506) 2469506..2470912 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
KIM92_RS11945 (2470991) 2470991..2471407 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KIM92_RS11950 (2471453) 2471453..2471629 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KIM92_RS11955 (2471851) 2471851..2472081 + 231 WP_000494244.1 YncJ family protein -
KIM92_RS11960 (2472173) 2472173..2474134 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KIM92_RS11965 (2474207) 2474207..2474743 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
KIM92_RS11970 (2474835) 2474835..2476010 + 1176 WP_001236319.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2476050..2477315 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T202634 WP_000813794.1 NZ_CP074680:c2471629-2471453 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T202634 NZ_CP099535:2691274-2691489 [Pantoea ananatis]
ATGAACAAAACCCTAACTAAAACTGATTATTTGATGCGACTGAGGCGCTGTCGTTCTATTGACACGCTGGAACGCGTGAT
TGAAAAAAATAAGTACGAATTATCCGATGAAGAATTAGCAGTATTTTACTCAGCAGCCGATCACCGCCTGGCTGAACTGA
CGATGAACAAGCTCTACGACAAAGTCCCGGTGTCAGTCTGGAAATTTGTACGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT202634 WP_001270286.1 NZ_CP074680:c2471407-2470991 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT202634 NZ_CP099535:2690872-2691249 [Pantoea ananatis]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACTTGTGTGAGAGTCTGTTTGACGAAAGCATGGC
GACATTAACTGACAGTCATCACGGCTGGGTTAATGATCCCACTGCCGAAAGCAATTTACAGCTAAACGATTTGATTGAGC
ACATTGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGACGAAGCACTGATTACCCAAATCGATGAATATCTT
GATGATACCTTTATGCTGTTTAGCAACTACAGTGTAAATACTCAGGATCTTCAGCGCTGGCAACGCTCAGCGAAACGTTT
ATTTAATGTGTTTACTGAAGAGTGCGCTTTTCTTCAACAGCCAAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References