Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 998570..999153 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag KIM92_RS04810 Protein ID WP_000254745.1
Coordinates 998818..999153 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KIM92_RS04805 Protein ID WP_000581937.1
Coordinates 998570..998818 (+) Length 83 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS04795 (994909) 994909..996210 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
KIM92_RS04800 (996258) 996258..998492 + 2235 WP_000226815.1 GTP pyrophosphokinase -
KIM92_RS04805 (998570) 998570..998818 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KIM92_RS04810 (998818) 998818..999153 + 336 WP_000254745.1 endoribonuclease MazF Toxin
KIM92_RS04815 (999224) 999224..1000015 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
KIM92_RS04820 (1000243) 1000243..1001880 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KIM92_RS04825 (1001968) 1001968..1003266 + 1299 WP_000036723.1 phosphopyruvate hydratase -
KIM92_RS04830 (1003322) 1003322..1003684 - 363 WP_000034929.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(10-109)

Antitoxin

(7-51)


Sequences


Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T202622 WP_000254745.1 NZ_CP074680:998818-999153 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T202622 NZ_CP099531:c4624949-4624815 [Xanthomonas sacchari]
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTCTCTGTGGGCCTGCTGGCCGGTTGCAACACCGTTGCCGGTGC
CGGCAAGGACGTGCAGAAGGCGGGCGAGAAGGTCCAAGACGCCGCCAAGAACTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT202622 WP_000581937.1 NZ_CP074680:998570-998818 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT202622 NZ_CP099531:c4625157-4625005 [Xanthomonas sacchari]
ATGAAGCGACTGACCACCCTGTTGATGCTGACCCTGTTTTGCGCTGGTGTGCTGACCGGCTGCAACACCGTTGCCGGCGC
CGGCAAGGACATGCAGAAGGCGGGCGAGAAGGTGGAAGACACCGCCAAGGATTGCAGCGCGGGCAAGTGCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3


Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References