Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | mazEF/PRK09907-MazE |
Location | 998570..999153 | Replicon | chromosome |
Accession | NZ_CP074680 | ||
Organism | Escherichia coli strain MEI008 |
Toxin (Protein)
Gene name | mazF | Uniprot ID | U9XFN8 |
Locus tag | KIM92_RS04810 | Protein ID | WP_000254745.1 |
Coordinates | 998818..999153 (+) | Length | 112 a.a. |
Antitoxin (Protein)
Gene name | mazE | Uniprot ID | S1P3W5 |
Locus tag | KIM92_RS04805 | Protein ID | WP_000581937.1 |
Coordinates | 998570..998818 (+) | Length | 83 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
KIM92_RS04795 (994909) | 994909..996210 | + | 1302 | WP_000046790.1 | 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD | - |
KIM92_RS04800 (996258) | 996258..998492 | + | 2235 | WP_000226815.1 | GTP pyrophosphokinase | - |
KIM92_RS04805 (998570) | 998570..998818 | + | 249 | WP_000581937.1 | type II toxin-antitoxin system antitoxin MazE | Antitoxin |
KIM92_RS04810 (998818) | 998818..999153 | + | 336 | WP_000254745.1 | endoribonuclease MazF | Toxin |
KIM92_RS04815 (999224) | 999224..1000015 | + | 792 | WP_001071648.1 | nucleoside triphosphate pyrophosphohydrolase | - |
KIM92_RS04820 (1000243) | 1000243..1001880 | + | 1638 | WP_000210878.1 | CTP synthase (glutamine hydrolyzing) | - |
KIM92_RS04825 (1001968) | 1001968..1003266 | + | 1299 | WP_000036723.1 | phosphopyruvate hydratase | - |
KIM92_RS04830 (1003322) | 1003322..1003684 | - | 363 | WP_000034929.1 | hypothetical protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 112 a.a. Molecular weight: 12158.08 Da Isoelectric Point: 8.4777
>T202622 WP_000254745.1 NZ_CP074680:998818-999153 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG
Download Length: 336 bp
>T202622 NZ_CP099531:c4624949-4624815 [Xanthomonas sacchari]
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTCTCTGTGGGCCTGCTGGCCGGTTGCAACACCGTTGCCGGTGC
CGGCAAGGACGTGCAGAAGGCGGGCGAGAAGGTCCAAGACGCCGCCAAGAACTGA
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTCTCTGTGGGCCTGCTGGCCGGTTGCAACACCGTTGCCGGTGC
CGGCAAGGACGTGCAGAAGGCGGGCGAGAAGGTCCAAGACGCCGCCAAGAACTGA
Antitoxin
Download Length: 83 a.a. Molecular weight: 9355.74 Da Isoelectric Point: 4.4665
>AT202622 WP_000581937.1 NZ_CP074680:998570-998818 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW
Download Length: 249 bp
>AT202622 NZ_CP099531:c4625157-4625005 [Xanthomonas sacchari]
ATGAAGCGACTGACCACCCTGTTGATGCTGACCCTGTTTTGCGCTGGTGTGCTGACCGGCTGCAACACCGTTGCCGGCGC
CGGCAAGGACATGCAGAAGGCGGGCGAGAAGGTGGAAGACACCGCCAAGGATTGCAGCGCGGGCAAGTGCTGA
ATGAAGCGACTGACCACCCTGTTGATGCTGACCCTGTTTTGCGCTGGTGTGCTGACCGGCTGCAACACCGTTGCCGGCGC
CGGCAAGGACATGCAGAAGGCGGGCGAGAAGGTGGAAGACACCGCCAAGGATTGCAGCGCGGGCAAGTGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A0E0XYM3 |
Antitoxin
Source | ID | Structure |
---|---|---|
PDB | 1UB4 | |
PDB | 5CQX | |
PDB | 5CQY | |
PDB | 1MVF | |
PDB | 2MRN | |
PDB | 2MRU | |
AlphaFold DB | A0A7U9LMB4 |