Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 286632..287432 Replicon chromosome
Accession NZ_CP074680
Organism Escherichia coli strain MEI008

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KIM92_RS01305 Protein ID WP_000342449.1
Coordinates 286905..287432 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KIM92_RS01300 Protein ID WP_001277108.1
Coordinates 286632..286898 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM92_RS01280 (282290) 282290..282958 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KIM92_RS01285 (282951) 282951..284009 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KIM92_RS01290 (284254) 284254..285108 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KIM92_RS01295 (285379) 285379..286482 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KIM92_RS01300 (286632) 286632..286898 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KIM92_RS01305 (286905) 286905..287432 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KIM92_RS01310 (287429) 287429..287812 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
KIM92_RS01315 (288236) 288236..289345 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KIM92_RS01320 (289393) 289393..290319 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KIM92_RS01325 (290316) 290316..291593 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
KIM92_RS01330 (291590) 291590..292357 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T202620 WP_000342449.1 NZ_CP074680:286905-287432 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T202620 NZ_CP099531:3790530-3790787 [Xanthomonas sacchari]
GTGACGCTGCAGTTCTCGGACAACGCCTGGGAGGATTATCTCTACTGGCAACAGGCCGACAAAAAGATGCTCAAGCGCAT
CAACGACCTGATCAAGGCCATTCAACGCGATCCCTTGCAGGGCATCGGCAAACCCGAACCGCTACGCCATGCCCTGGCCG
GCTATTGGTCGCGACGCGTCAACGACGAACACCGGATCGTCTATAAGGTCGAGCATGGCGTCCTGTTGATTGCACAACTG
CGGTATCACTACGTGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT202620 WP_001277108.1 NZ_CP074680:286632-286898 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT202620 NZ_CP099531:3790282-3790533 [Xanthomonas sacchari]
ATGGACACCATCACCTACAGCGCCGCACGTGCCGCTCTCGCGGACACCATGGACCGCGTGGTCAACAACCACGAGCCGGT
GATCATTACGCGCAGCCGCGAGCAAGCCGTCGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAAGAAACCGCTTATT
TGCTGCGTAGCCCGAAGAACGCGCAGCGCCTGCTGGAATCCATCGCACAACTCGAATCCGGTCGCGGCAAGGCGCGGGAA
CTGAGCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References