Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 154021..154547 Replicon plasmid pMEI009_1
Accession NZ_CP074678
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KIM99_RS25650 Protein ID WP_000323025.1
Coordinates 154021..154308 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag KIM99_RS25655 Protein ID WP_004196370.1
Coordinates 154308..154547 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS25620 (KIM99_25575) 149177..149572 - 396 Protein_151 hypothetical protein -
KIM99_RS25625 (KIM99_25580) 149630..150598 - 969 WP_074449053.1 IS5-like element IS903B family transposase -
KIM99_RS25630 (KIM99_25585) 151499..152611 - 1113 WP_001300563.1 IS4-like element IS421 family transposase -
KIM99_RS25635 152690..152788 - 99 WP_159224797.1 Hok/Gef family protein -
KIM99_RS25640 152763..152819 - 57 Protein_155 modulator protein -
KIM99_RS25645 (KIM99_25595) 152920..153954 + 1035 Protein_156 IS481 family transposase -
KIM99_RS25650 (KIM99_25600) 154021..154308 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KIM99_RS25655 (KIM99_25605) 154308..154547 - 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KIM99_RS25660 154572..154670 + 99 Protein_159 hypothetical protein -
KIM99_RS25665 (KIM99_25610) 154798..155163 + 366 WP_009651956.1 hypothetical protein -
KIM99_RS25670 (KIM99_25615) 155207..155944 + 738 WP_008460272.1 HupE/UreJ family protein -
KIM99_RS25675 (KIM99_25620) 155958..156647 + 690 WP_004196322.1 hypothetical protein -
KIM99_RS25680 (KIM99_25625) 156678..158054 - 1377 Protein_163 chromate efflux transporter -
KIM99_RS25685 (KIM99_25630) 158011..158988 - 978 WP_004196334.1 chromate resistance protein -
KIM99_RS25690 (KIM99_25635) 159018..159210 + 193 Protein_165 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..203030 203030


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T202618 WP_000323025.1 NZ_CP074678:c154308-154021 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T202618 NZ_CP099530:c4706176-4706024 [Xanthomonas sacchari]
ATGAAGCGACTGACCACCCTGTTGATGCTGACCCTGTTTTGCGCTGGTGTGCTGACCGGCTGCAACACCGTTGCCGGCGC
CGGCAAGGACATGCAGAAGGCGGGCGAGAAGGTGGAAGACACCGCCAAGGATTGCAGCGCGGGTAAGTGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT202618 WP_004196370.1 NZ_CP074678:c154547-154308 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT202618 NZ_CP099530:c4705968-4705834 [Xanthomonas sacchari]
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGGCCGGTTGCAACACCGTTGCCGGTGC
CGGCAAGGACGTGCAGAAGGCGGGCGAGAAGGTCCAAGACGCCGCCAAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References