Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 65510..66246 Replicon plasmid pMEI009_1
Accession NZ_CP074678
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag KIM99_RS25210 Protein ID WP_004098919.1
Coordinates 65510..65992 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A455TK27
Locus tag KIM99_RS25215 Protein ID WP_020804426.1
Coordinates 65980..66246 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS25195 (KIM99_25155) 61642..62274 + 633 WP_001567369.1 hypothetical protein -
KIM99_RS25200 (KIM99_25160) 62303..63706 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
KIM99_RS25205 (KIM99_25165) 63818..65350 + 1533 WP_009310015.1 IS3-like element ISKpn38 family transposase -
KIM99_RS25210 (KIM99_25170) 65510..65992 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
KIM99_RS25215 (KIM99_25175) 65980..66246 - 267 WP_020804426.1 DUF1778 domain-containing protein Antitoxin
KIM99_RS25220 (KIM99_25180) 66422..66676 - 255 WP_032425571.1 hypothetical protein -
KIM99_RS25225 (KIM99_25185) 66860..67840 - 981 WP_000082736.1 IS5-like element ISEc68 family transposase -
KIM99_RS25230 (KIM99_25190) 67953..68210 - 258 WP_004098928.1 hypothetical protein -
KIM99_RS25235 (KIM99_25195) 68259..68462 - 204 WP_004150739.1 HHA domain-containing protein -
KIM99_RS25240 (KIM99_25200) 68523..69017 - 495 WP_016528789.1 hypothetical protein -
KIM99_RS25245 (KIM99_25205) 69048..69614 - 567 WP_042922197.1 hypothetical protein -
KIM99_RS25250 (KIM99_25210) 69611..69874 - 264 WP_004118691.1 hypothetical protein -
KIM99_RS25255 (KIM99_25215) 70188..70367 + 180 WP_004118349.1 type II toxin-antitoxin system ParD family antitoxin -
KIM99_RS25260 70489..70651 + 163 Protein_79 type II toxin-antitoxin system RelE/ParE family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..203030 203030
- inside IScluster/Tn - - 38209..67840 29631


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T202617 WP_004098919.1 NZ_CP074678:c65992-65510 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T202617 NZ_CP099530:c4705968-4705834 [Xanthomonas sacchari]
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGGCCGGTTGCAACACCGTTGCCGGTGC
CGGCAAGGACGTGCAGAAGGCGGGCGAGAAGGTCCAAGACGCCGCCAAGAACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10124.47 Da        Isoelectric Point: 4.3108

>AT202617 WP_020804426.1 NZ_CP074678:c66246-65980 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINELL
ARKPQWDV

Download         Length: 267 bp

>AT202617 NZ_CP099530:c4706176-4706024 [Xanthomonas sacchari]
ATGAAGCGACTGACCACCCTGTTGATGCTGACCCTGTTTTGCGCTGGTGTGCTGACCGGCTGCAACACCGTTGCCGGCGC
CGGCAAGGACATGCAGAAGGCGGGCGAGAAGGTGGAAGACACCGCCAAGGATTGCAGCGCGGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A455TK27

References