Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 18953..19596 Replicon plasmid pMEI009_1
Accession NZ_CP074678
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag KIM99_RS24965 Protein ID WP_020804312.1
Coordinates 19180..19596 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KIM99_RS24960 Protein ID WP_001261276.1
Coordinates 18953..19183 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS24925 (KIM99_24895) 14735..14992 + 258 WP_009310077.1 hypothetical protein -
KIM99_RS24930 (KIM99_24900) 15050..15829 - 780 WP_023287113.1 site-specific integrase -
KIM99_RS24935 (KIM99_24905) 16027..17043 - 1017 WP_009309921.1 hypothetical protein -
KIM99_RS24940 (KIM99_24910) 17077..17412 - 336 WP_009309920.1 hypothetical protein -
KIM99_RS24945 (KIM99_24915) 17462..17602 - 141 WP_162898808.1 hypothetical protein -
KIM99_RS24950 (KIM99_24920) 17831..18136 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
KIM99_RS24955 (KIM99_24925) 18138..18356 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
KIM99_RS24960 (KIM99_24930) 18953..19183 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KIM99_RS24965 (KIM99_24935) 19180..19596 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
KIM99_RS24970 (KIM99_24940) 19637..20515 - 879 WP_032427228.1 restriction endonuclease -
KIM99_RS24975 (KIM99_24945) 21177..22454 + 1278 WP_009309915.1 HlyD family secretion protein -
KIM99_RS24980 (KIM99_24950) 22444..24552 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..203030 203030
- flank IS/Tn - - 13628..14608 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T202616 WP_020804312.1 NZ_CP074678:19180-19596 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T202616 NZ_CP099530:3833556-3833813 [Xanthomonas sacchari]
GTGACGCTGCAGTTCTCGGACAACGCCTGGGAGGATTATCTCTACTGGCAACAGGCCGACAAAAAGATGCTCAAGCGCAT
CAACGACCTGATCAAGGCCATTCAACGCGATCCCTTCCAGGGCATCGGCAAGCCCGAACCGCTACGCCATGCCCTGGCCG
GCTATTGGTCGCGACGCATCAACGACGAACACCGGATCGTCTATAAGGTCGAGCATGGCATTCTGCTGATTGCACAAGTG
CGATATCACTACGTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT202616 WP_001261276.1 NZ_CP074678:18953-19183 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT202616 NZ_CP099530:3833308-3833559 [Xanthomonas sacchari]
ATGGACACCATCACCTACAGCGCCGCACGTGCCGCTCTCGCGGACACCATGGACCGCGTGGTCAACAACCACGAGCCGGT
GATCATCACGCGCAGCCGCGAGCAAGCCGTTGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTATC
TGCTGCGCAGCCCGAAGAACGCGCAGCGCTTGCTGGAATCCATCGCCCAACTCGAATCCGGTCGCGGCAAGGCGCGGGAA
CTGAGCGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References