Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 17831..18356 Replicon plasmid pMEI009_1
Accession NZ_CP074678
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag KIM99_RS24950 Protein ID WP_004197633.1
Coordinates 17831..18136 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag KIM99_RS24955 Protein ID WP_006788213.1
Coordinates 18138..18356 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS24920 (KIM99_24890) 13628..14608 - 981 WP_000019449.1 IS5-like element ISKpn26 family transposase -
KIM99_RS24925 (KIM99_24895) 14735..14992 + 258 WP_009310077.1 hypothetical protein -
KIM99_RS24930 (KIM99_24900) 15050..15829 - 780 WP_023287113.1 site-specific integrase -
KIM99_RS24935 (KIM99_24905) 16027..17043 - 1017 WP_009309921.1 hypothetical protein -
KIM99_RS24940 (KIM99_24910) 17077..17412 - 336 WP_009309920.1 hypothetical protein -
KIM99_RS24945 (KIM99_24915) 17462..17602 - 141 WP_162898808.1 hypothetical protein -
KIM99_RS24950 (KIM99_24920) 17831..18136 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
KIM99_RS24955 (KIM99_24925) 18138..18356 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KIM99_RS24960 (KIM99_24930) 18953..19183 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin -
KIM99_RS24965 (KIM99_24935) 19180..19596 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
KIM99_RS24970 (KIM99_24940) 19637..20515 - 879 WP_032427228.1 restriction endonuclease -
KIM99_RS24975 (KIM99_24945) 21177..22454 + 1278 WP_009309915.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..203030 203030
- flank IS/Tn - - 13628..14608 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T202615 WP_004197633.1 NZ_CP074678:c18136-17831 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T202615 NZ_CP099530:3833308-3833559 [Xanthomonas sacchari]
ATGGACACCATCACCTACAGCGCCGCACGTGCCGCTCTCGCGGACACCATGGACCGCGTGGTCAACAACCACGAGCCGGT
GATCATCACGCGCAGCCGCGAGCAAGCCGTTGTCATGCTGTCGCTGGAGGACTACAAGGCGATGGAGGAAACCGCCTATC
TGCTGCGCAGCCCGAAGAACGCGCAGCGCTTGCTGGAATCCATCGCCCAACTCGAATCCGGTCGCGGCAAGGCGCGGGAA
CTGAGCGAGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT202615 WP_006788213.1 NZ_CP074678:c18356-18138 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT202615 NZ_CP099530:3833556-3833813 [Xanthomonas sacchari]
GTGACGCTGCAGTTCTCGGACAACGCCTGGGAGGATTATCTCTACTGGCAACAGGCCGACAAAAAGATGCTCAAGCGCAT
CAACGACCTGATCAAGGCCATTCAACGCGATCCCTTCCAGGGCATCGGCAAGCCCGAACCGCTACGCCATGCCCTGGCCG
GCTATTGGTCGCGACGCATCAACGACGAACACCGGATCGTCTATAAGGTCGAGCATGGCATTCTGCTGATTGCACAAGTG
CGATATCACTACGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References