Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5038785..5039410 Replicon chromosome
Accession NZ_CP074677
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KIM99_RS24500 Protein ID WP_002882817.1
Coordinates 5038785..5039168 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KIM99_RS24505 Protein ID WP_004150355.1
Coordinates 5039168..5039410 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS24485 (5036151) 5036151..5037053 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KIM99_RS24490 (5037050) 5037050..5037685 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KIM99_RS24495 (5037682) 5037682..5038611 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KIM99_RS24500 (5038785) 5038785..5039168 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KIM99_RS24505 (5039168) 5039168..5039410 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KIM99_RS24510 (5039615) 5039615..5040532 + 918 WP_004178029.1 alpha/beta hydrolase -
KIM99_RS24515 (5040546) 5040546..5041487 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KIM99_RS24520 (5041532) 5041532..5041969 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KIM99_RS24525 (5041966) 5041966..5042826 - 861 WP_002882807.1 virulence factor BrkB family protein -
KIM99_RS24530 (5042820) 5042820..5043419 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202614 WP_002882817.1 NZ_CP074677:c5039168-5038785 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202614 NZ_CP099529:c4672712-4672560 [Xanthomonas sacchari]
ATGAAGCGACTGACCACCCTGTTGATGCTGACCCTGTTTTGCGCTGGTGTGCTGACCGGCTGCAACACCGTTGCCGGCGC
CGGCAAGGACATGCAGAAGGCGGGCGAGAAGGTGGAAGACACCGCCAAGGATTGCAGCGCGGGCAAGTGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202614 WP_004150355.1 NZ_CP074677:c5039410-5039168 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202614 NZ_CP099529:c4672504-4672370 [Xanthomonas sacchari]
ATGAAGCGTACGTTTGCGTGGATGCTGCTGGCGATGTTCTCGGTGGGCCTGCTGGCCGGTTGCAACACCGTTGCCGGTGC
CGGCAAGGACGTGCAGAAGGCGGGCGAGAAGGTCCAAGACGCCGCCAAGAACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References