Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3825211..3825830 Replicon chromosome
Accession NZ_CP074677
Organism Klebsiella pneumoniae strain MEI009

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KIM99_RS18650 Protein ID WP_002892050.1
Coordinates 3825612..3825830 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KIM99_RS18645 Protein ID WP_002892066.1
Coordinates 3825211..3825585 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM99_RS18635 (3820363) 3820363..3821556 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KIM99_RS18640 (3821579) 3821579..3824725 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KIM99_RS18645 (3825211) 3825211..3825585 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KIM99_RS18650 (3825612) 3825612..3825830 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KIM99_RS18655 (3825989) 3825989..3826555 + 567 WP_002892030.1 maltose O-acetyltransferase -
KIM99_RS18660 (3826527) 3826527..3826667 - 141 WP_004147370.1 hypothetical protein -
KIM99_RS18665 (3826688) 3826688..3827158 + 471 WP_002892026.1 YlaC family protein -
KIM99_RS18670 (3827133) 3827133..3828584 - 1452 WP_009307955.1 PLP-dependent aminotransferase family protein -
KIM99_RS18675 (3828684) 3828684..3829382 + 699 WP_002892021.1 GNAT family protein -
KIM99_RS18680 (3829379) 3829379..3829519 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KIM99_RS18685 (3829519) 3829519..3829782 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202609 WP_002892050.1 NZ_CP074677:3825612-3825830 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202609 NZ_CP099523:c186170-185883 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT202609 WP_002892066.1 NZ_CP074677:3825211-3825585 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202609 NZ_CP099523:c186409-186170 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References