Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-PumB/upstrm_HI1419-dnstrm_HI1420
Location 1020662..1021257 Replicon chromosome
Accession NZ_CP074676
Organism Pseudomonas qingdaonensis strain S-1

Toxin (Protein)


Gene name relE Uniprot ID A0A1Y3KHK4
Locus tag KH389_RS04565 Protein ID WP_043864446.1
Coordinates 1020662..1020955 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1Y3KHQ4
Locus tag KH389_RS04570 Protein ID WP_043864447.1
Coordinates 1020958..1021257 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH389_RS04545 (KH389_04545) 1016675..1017820 - 1146 WP_043864442.1 FMNH2-dependent alkanesulfonate monooxygenase -
KH389_RS04550 (KH389_04550) 1017817..1018395 - 579 WP_043864443.1 FMN reductase -
KH389_RS04555 (KH389_04555) 1018634..1019416 - 783 WP_213606962.1 helix-turn-helix transcriptional regulator -
KH389_RS04560 (KH389_04560) 1019510..1020418 + 909 WP_213606963.1 EamA family transporter -
KH389_RS04565 (KH389_04565) 1020662..1020955 + 294 WP_043864446.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KH389_RS04570 (KH389_04570) 1020958..1021257 + 300 WP_043864447.1 putative addiction module antidote protein Antitoxin
KH389_RS04575 (KH389_04575) 1021476..1022378 - 903 WP_043864448.1 transporter substrate-binding domain-containing protein -
KH389_RS04580 (KH389_04580) 1022409..1022759 - 351 WP_043864449.1 RidA family protein -
KH389_RS04585 (KH389_04585) 1022785..1024026 - 1242 WP_213606965.1 D-amino acid dehydrogenase -
KH389_RS04590 (KH389_04590) 1024134..1025039 + 906 WP_213606967.1 LysR substrate-binding domain-containing protein -
KH389_RS04595 (KH389_04595) 1025495..1026055 + 561 WP_143516664.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-96)

Antitoxin

Uncharacterized protein HI1420

(5-95)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10480.14 Da        Isoelectric Point: 10.6099

>T202593 WP_043864446.1 NZ_CP074676:1020662-1020955 [Pseudomonas qingdaonensis]
VIYSIEQTLAFSGWLLALRDVRARIAIGRRIERALAGNLGDTKAVGDGVSEMRIDSGPGYRVYFTVRSGVAILLLTGGDK
SSQAADIRMAKRLAKEV

Download         Length: 294 bp

>T202593 NZ_CP099513:c3640395-3640042 [Klebsiella sp. ZYC-1]
GTGTGGATGATTAAAACAACGGATACGTTTGAGCGCTGGTTTACCTCGTTCAACGATACCGATAGGGCCAGCGTGCTTGC
AGCGTTGTTGGTCCTGCGAGAAAAGGGGCCGGGTTTGTCCAGACCTTATGCCGATACGCTGAGAGGCTCCCGCTACAGCG
ACATGAAAGAGTTGCGTATTCAGAGCCGGGGCGAGCCAATACGGGCATTTTTCGCTTTTGACCCGGCCCGTACCGGCATT
GTGCTTTGTGCCGGTAATAAGGTTGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCTGTTGCTGAGCGGGAATTCAC
GAACTGGCTGAAGACATTCAAAGAGAAGGAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10599.26 Da        Isoelectric Point: 5.6850

>AT202593 WP_043864447.1 NZ_CP074676:1020958-1021257 [Pseudomonas qingdaonensis]
MSVTLIPFDMAALLDSDEAISEYLSQVLAEGDSDELLRAIGYVAKARGMSLVAKETGLGRESLYKAFAPGAKPRFDTVLK
VMHALGIELCARSHRGASV

Download         Length: 300 bp

>AT202593 NZ_CP099513:c3640037-3639735 [Klebsiella sp. ZYC-1]
ATGGGCAGAACGTTGGAACAGCTGATGGCGGATGAAAAACCGGAAGTCGTGGCTGACGCCCGGGCTATGGCGGCGGACAT
CCTGCTCAACATTCACCTTGCAGAACTGCGTGAGAAAGTACAGAAAACGCAGGTCGAAATGGCGCAGGCGCTGGGCATCA
GGCAACCTACCGTTGCAGGCATGGAAAAACCAGGCCGTGACCTGAAGCTTTCCACGCTTAAACGCTACGTTGAAGCCACC
GGCGGCAAGCTACGTCTTGATGTTGAGTTGCCGGATGGTTCTCACTACGGATTTGAACTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Y3KHK4

Antitoxin

Source ID Structure
AlphaFold DB A0A1Y3KHQ4

References