Detailed information of TA system TA202591

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 298154..298791
• Replicon: chromosome
• Accession: NZ_CP074676
• Organism: Pseudomonas qingdaonensis strain S-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KH389_RS01385
• Protein ID: WP_213606734.1
• Coordinates: 298154..298336 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KH389_RS01390
• Protein ID: WP_213606736.1
• Coordinates: 298366..298791 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KH389_RS01360 (KH389_01360)	293803..294630	+	828	WP_213606732.1	DUF2314 domain-containing protein	-
KH389_RS01365 (KH389_01365)	294944..295231	-	288	WP_043859808.1	HigA family addiction module antitoxin	-
KH389_RS01370 (KH389_01370)	295244..295522	-	279	WP_043859809.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KH389_RS01375 (KH389_01375)	296433..297398	+	966	WP_213606733.1	hypothetical protein	-
KH389_RS01380 (KH389_01380)	297446..297994	-	549	Protein_269	hypothetical protein	-
KH389_RS01385 (KH389_01385)	298154..298336	+	183	WP_213606734.1	type II toxin-antitoxin system HicA family toxin	Toxin
KH389_RS01390 (KH389_01390)	298366..298791	+	426	WP_213606736.1	helix-turn-helix domain-containing protein	Antitoxin
KH389_RS01395 (KH389_01395)	299099..300427	+	1329	WP_213606737.1	site-specific integrase	-
KH389_RS01400 (KH389_01400)	300441..301127	+	687	WP_213606738.1	hypothetical protein	-
KH389_RS01405 (KH389_01405)	301197..301469	+	273	WP_213606739.1	helix-turn-helix domain-containing protein	-
KH389_RS01410 (KH389_01410)	301471..301788	+	318	WP_213606740.1	hypothetical protein	-
KH389_RS01415 (KH389_01415)	302112..302393	+	282	WP_213606741.1	hypothetical protein	-
KH389_RS01420 (KH389_01420)	302386..303273	+	888	WP_213606742.1	toprim domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	266437..325871	59434

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

• Cro/C1-type helix-turn-helix domain (82-112)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6929.03 Da
• Isoelectric Point: 10.8497

>T202591 WP_213606734.1 NZ_CP074676:298154-298336 [Pseudomonas qingdaonensis]
MKYSEFRRWLRAHGVTFVPAKGSHFKVYLGNRQTIFPDHGAKEISEGLRRKIIKDLGLGD

Nucleotide

• Length: 183 bp

>T202591 NZ_CP099513:c1313330-1313112 [Klebsiella sp. ZYC-1]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGCTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCCGATAATGAACTGGCGGTATTTTACTCAGCGGCCGACCATCGTCTTGCCGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCTTCAGTCTGGAAATTTATCCGCTAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15412.69 Da
• Isoelectric Point: 4.7523

>AT202591 WP_213606736.1 NZ_CP074676:298366-298791 [Pseudomonas qingdaonensis]
MYDYPVTVHEEEGGPWVSCADVPEMSSAGDTVEEALFDAVDALESALSFYVDQRIAIPLPSPPALGQPVVRLPALTAAKA
ALWNTMIKQKVTKTELARRLGVNRPQVDRLVDLLHRSKIEQVEHALQVLGQRIELSVVAAS

Nucleotide

• Length: 426 bp

>AT202591 NZ_CP099513:c1313731-1313357 [Klebsiella sp. ZYC-1]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCACGGCTGGGTTAATGACCCGACCTCGGCTGTCAATCTTCAACTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGATTACTCAGATTGACGAATATCTG
GATGACACTTTCGTCTTGTTCAGCAACTACGGCATTAACAGTGCGGATCTGCAGAAATGGCGCAAATCTGGCAATCGGTT
ATTCCGCTGCTTTGTCAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAG