Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 16150..16675 Replicon plasmid pCFSAN008104
Accession NZ_CP074662
Organism Salmonella enterica subsp. enterica serovar Enteritidis strain CFSAN008104

Toxin (Protein)


Gene name ccdB Uniprot ID I3W3D5
Locus tag A9D71_RS22650 Protein ID WP_001159863.1
Coordinates 16150..16455 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID M7S5D0
Locus tag A9D71_RS22655 Protein ID WP_000813641.1
Coordinates 16457..16675 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9D71_RS22610 (A9D71_22595) 11529..12089 - 561 WP_000900095.1 inverse autotransporter beta domain-containing protein -
A9D71_RS22615 (A9D71_22600) 12245..12532 + 288 WP_071530243.1 hypothetical protein -
A9D71_RS22620 (A9D71_22605) 12809..13294 - 486 WP_000905606.1 membrane protein -
A9D71_RS22625 (A9D71_22610) 13288..13776 - 489 WP_001075507.1 CaiF/GrlA family transcriptional regulator -
A9D71_RS22630 (A9D71_22615) 13801..14514 - 714 WP_000545756.1 EAL domain-containing protein -
A9D71_RS22635 (A9D71_22620) 14523..15305 - 783 WP_000082169.1 site-specific integrase -
A9D71_RS22640 (A9D71_22625) 15340..15861 - 522 WP_000198608.1 hypothetical protein -
A9D71_RS22645 (A9D71_22630) 15858..16148 - 291 WP_001266176.1 hypothetical protein -
A9D71_RS22650 (A9D71_22635) 16150..16455 - 306 WP_001159863.1 type II toxin-antitoxin system toxin CcdB Toxin
A9D71_RS22655 (A9D71_22640) 16457..16675 - 219 WP_000813641.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
A9D71_RS22660 (A9D71_22645) 17351..17872 + 522 WP_077681952.1 hypothetical protein -
A9D71_RS22665 (A9D71_22650) 17916..18344 - 429 Protein_24 hypothetical protein -
A9D71_RS22670 (A9D71_22655) 18356..18651 + 296 Protein_25 cytoplasmic protein -
A9D71_RS22675 (A9D71_22660) 19145..20134 + 990 WP_000461382.1 RepB family plasmid replication initiator protein -
A9D71_RS22680 (A9D71_22665) 20503..20622 - 120 Protein_27 recombinase -
A9D71_RS22685 (A9D71_22670) 20675..21061 - 387 WP_000751876.1 YgiW/YdeI family stress tolerance OB fold protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - spvB / spvC / fdeC / pefB / pefA / pefC / rck / rck 1..58711 58711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11587.48 Da        Isoelectric Point: 6.9787

>T202563 WP_001159863.1 NZ_CP074662:c16455-16150 [Salmonella enterica subsp. enterica serovar Enteritidis]
MQFKVYTCKRESRYRLFVDVQSDIIDTPGRRMAVPLVSARLLSEKVPRDLYPVMHIGDEPYRLLTTDMTSVPATVIGEEV
ADLSLRENDIKNAINLMFRGI

Download         Length: 306 bp

>T202563 NZ_CP099506:c517925-517737 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8314.16 Da        Isoelectric Point: 4.5203

>AT202563 WP_000813641.1 NZ_CP074662:c16675-16457 [Salmonella enterica subsp. enterica serovar Enteritidis]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADDNRNW

Download         Length: 219 bp

>AT202563 NZ_CP099506:c518213-517953 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I3W3D5

Antitoxin

Source ID Structure
AlphaFold DB A0A656ICA6

References