Detailed information of TA system TA202533

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4176179..4176837
• Replicon: chromosome
• Accession: NZ_CP074606
• Organism: Salmonella enterica subsp. enterica serovar Newport str. CFSAN000827

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A3V9WZG5
• Locus tag: DLM99_RS20395
• Protein ID: WP_000854676.1
• Coordinates: 4176179..4176475 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A3V9WZC1
• Locus tag: DLM99_RS20400
• Protein ID: WP_031607465.1
• Coordinates: 4176496..4176837 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLM99_RS20380 (4172407)	4172407..4173123	+	717	WP_000532939.1	YebC/PmpR family DNA-binding transcriptional regulator	-
DLM99_RS20385 (4173601)	4173601..4174476	-	876	WP_000199883.1	HNH endonuclease	-
DLM99_RS20390 (4174876)	4174876..4175865	-	990	WP_077906441.1	restriction endonuclease	-
DLM99_RS20395 (4176179)	4176179..4176475	-	297	WP_000854676.1	TA system toxin CbtA family protein	Toxin
DLM99_RS20400 (4176496)	4176496..4176837	-	342	WP_031607465.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DLM99_RS20405 (4176848)	4176848..4177390	-	543	WP_001098987.1	DNA repair protein RadC	-
DLM99_RS20410 (4177403)	4177403..4177846	-	444	WP_001025651.1	antirestriction protein	-
DLM99_RS20415 (4177877)	4177877..4178698	-	822	WP_001234409.1	DUF932 domain-containing protein	-
DLM99_RS20420 (4178797)	4178797..4179028	-	232	Protein_4025	DUF905 domain-containing protein	-
DLM99_RS20425 (4179100)	4179100..4179549	-	450	WP_000734139.1	IrmA family protein	-
DLM99_RS20430 (4179546)	4179546..4180001	-	456	WP_000754598.1	hypothetical protein	-
DLM99_RS20435 (4180040)	4180040..4180675	-	636	WP_000772023.1	hypothetical protein	-
DLM99_RS20440 (4180672)	4180672..4181385	-	714	WP_000075763.1	sporulation transcriptional regulator SpoIIID	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4166927..4195571	28644

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-98)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11081.81 Da
• Isoelectric Point: 6.4533

>T202533 WP_000854676.1 NZ_CP074606:c4176475-4176179 [Salmonella enterica subsp. enterica serovar Newport str.]
MKTLPATNLQAAKLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDATVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRAR

Nucleotide

• Length: 297 bp

>T202533 NZ_CP099495:1132553-1132741 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12659.30 Da
• Isoelectric Point: 6.4715

>AT202533 WP_031607465.1 NZ_CP074606:c4176837-4176496 [Salmonella enterica subsp. enterica serovar Newport str.]
MKSTDSENDSFLKWGLNRNVTPCFGACLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT202533 NZ_CP099495:1132265-1132525 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A3V9WZG5

Antitoxin

• AlphaFold DB: A0A3V9WZC1