Detailed information of TA system TA202528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3521775..3522395
• Replicon: chromosome
• Accession: NZ_CP074606
• Organism: Salmonella enterica subsp. enterica serovar Newport str. CFSAN000827

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: DLM99_RS17390
• Protein ID: WP_001280991.1
• Coordinates: 3522177..3522395 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: DLM99_RS17385
• Protein ID: WP_000344807.1
• Coordinates: 3521775..3522149 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLM99_RS17375 (3516914)	3516914..3518107	+	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
DLM99_RS17380 (3518130)	3518130..3521279	+	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
DLM99_RS17385 (3521775)	3521775..3522149	+	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
DLM99_RS17390 (3522177)	3522177..3522395	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
DLM99_RS17395 (3522574)	3522574..3523125	+	552	WP_001278792.1	maltose O-acetyltransferase	-
DLM99_RS17400 (3523242)	3523242..3523712	+	471	WP_000136181.1	YlaC family protein	-
DLM99_RS17405 (3523768)	3523768..3523908	-	141	WP_001197749.1	type B 50S ribosomal protein L36	-
DLM99_RS17410 (3523914)	3523914..3524174	-	261	WP_000801415.1	type B 50S ribosomal protein L31	-
DLM99_RS17415 (3524399)	3524399..3525949	+	1551	WP_000213128.1	EAL domain-containing protein	-
DLM99_RS17425 (3526180)	3526180..3526569	+	390	WP_000961285.1	MGMT family protein	-
DLM99_RS17430 (3526602)	3526602..3527171	-	570	WP_000779802.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T202528 WP_001280991.1 NZ_CP074606:3522177-3522395 [Salmonella enterica subsp. enterica serovar Newport str.]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T202528 NZ_CP099490:c3547666-3547418 [Ornithinimicrobium cryptoxanthini]
ATGTCCATCACCGCAAGCGAGGCCCGCAAGCGCCTCTTCCCCCTGATCGAGCAGGTCAACGATGACCGAGCTCCAGTGGA
GATCACCTCGAAGCGAGGCAACGCGGTACTCATGTCAGCCGAGGACTACTCGGCTTGGCAGGAGACGGCCTATCTGTTTC
GCTCTCCCGCCAACGCACGGAGACTGCTTGATGCCATGGAGGCGGTGGACCGCGGCGAAGGGGCTGAACGCGAACTTGAT
CGCACATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT202528 WP_000344807.1 NZ_CP074606:3521775-3522149 [Salmonella enterica subsp. enterica serovar Newport str.]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT202528 NZ_CP099490:c3547421-3547161 [Ornithinimicrobium cryptoxanthini]
ATGAGGCCCGTCTTCGCGCCTCAGGCGTGGGAGGACTACCTCCACTGGCAACGCATCGATCGGACCGTGGTGAAGCGGAT
CAACCGGCTGATCGATGACGCGACTCGGGACCCTTTCGCCGGAGTCGGCAAACCCGAGCCACTGAAGTACGGGCTGGCTC
ATGCCTGGTCGCGGCGCATCAACGAGGAGCACCGGTTGGTCTACGTGGTCCGAGACGATGACCTCGTCCTCCTACAAGCC
CGCTACCACTACACGAAGTAG

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0