Detailed information of TA system TA202516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 307197..307783
• Replicon: chromosome
• Accession: NZ_CP074606
• Organism: Salmonella enterica subsp. enterica serovar Newport str. CFSAN000827

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A3V9X0E4
• Locus tag: DLM99_RS01400
• Protein ID: WP_000174964.1
• Coordinates: 307415..307783 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: V7IU48
• Locus tag: DLM99_RS01395
• Protein ID: WP_001522145.1
• Coordinates: 307197..307418 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLM99_RS01370 (302218)	302218..303327	+	1110	WP_000822976.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
DLM99_RS01375 (303387)	303387..304313	+	927	WP_000003007.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
DLM99_RS01380 (304310)	304310..305587	+	1278	WP_000803764.1	branched chain amino acid ABC transporter permease LivM	-
DLM99_RS01385 (305584)	305584..306351	+	768	WP_000082081.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
DLM99_RS01390 (306353)	306353..307066	+	714	WP_000416114.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
DLM99_RS01395 (307197)	307197..307418	+	222	WP_001522145.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DLM99_RS01400 (307415)	307415..307783	+	369	WP_000174964.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
DLM99_RS01405 (308042)	308042..309358	+	1317	WP_000624747.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
DLM99_RS01410 (309463)	309463..310350	+	888	WP_000094074.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
DLM99_RS01415 (310347)	310347..311192	+	846	WP_000572196.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
DLM99_RS01420 (311195)	311195..312265	+	1071	WP_000907839.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	304310..313002	8692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-114)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13601.91 Da
• Isoelectric Point: 6.7252

>T202516 WP_000174964.1 NZ_CP074606:307415-307783 [Salmonella enterica subsp. enterica serovar Newport str.]
MTLQLISAEEIIQFHDRLLRVTPGVTGMPDPGRAEALMYRVLNQIEYEGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLAANPDFVDMTVDAAAGRLTLEQIVVRLRA

Nucleotide

• Length: 369 bp

>T202516 NZ_CP099489:4417668-4418057 [Ornithinimicrobium sp. HY1793]
ATGACCTATCTCCTAGACACCCACGCGGTGCTCTGGGCACTCACCGACCCCGCGCGTCTGGGGCCCGCCGCGCGCCAGAC
CATCACGGCCCGCTCCACCCGACTGGCCGTCTCAGCAGCAACGGCGTGGGAGATCTCCACCAAGCAGCGCATCGGCAAAC
TTCCCCAGGCGGACGCCCTCGTCGGCGGATACTCCCGGCATCTCGATCGGTTGGGTGCCATCAGACTGCCCATCGACGAG
GAACACGCGCTGCTCGCGGGACGGCTGGATTGGTCTCATCGCGATCCGTTTGATCGTGTGTTTGCCGCCCAAGCCATGCT
GGAGTCCCTCACGCTCGTGACGAACGACTCGGCCTTCGCGGAGCTCAACGGGGTCGCCACGCTCTGGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8166.17 Da
• Isoelectric Point: 5.0775

>AT202516 WP_001522145.1 NZ_CP074606:307197-307418 [Salmonella enterica subsp. enterica serovar Newport str.]
MRTVNYSEARQNLAEVLESAVTAGPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGNELRELADK

Nucleotide

• Length: 222 bp

>AT202516 NZ_CP099489:4417441-4417671 [Ornithinimicrobium sp. HY1793]
ATGGAAACGGTCAAGGTGCAATACGCCAAGACGCACTTGTCCGCGCTGCTGGCAGCAGTCGAGCGGGGCGAAGAGATCGT
CATCGCGCGCGGGGACCGCCCCGTCGCCCGGCTGTCCCCGATCGATGGCCAGGACGACCGCGAGCTGGGCTTCGTCGCCT
ACTCAGTGCCTGACGAGTTCTTCGACCCTCTGCCAGAGGAGGAACTCGCTGTGTGGGAGGGCGCGGGATGA

Structures

Toxin

• AlphaFold DB: A0A3V9X0E4