Detailed information of TA system TA202480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 193011..193750
• Replicon: chromosome
• Accession: NZ_CP074584
• Organism: Enterobacter asburiae strain L1 delta-T1RM

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: KIJ47_RS01035
• Protein ID: WP_029739942.1
• Coordinates: 193265..193750 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A157GCY0
• Locus tag: KIJ47_RS01030
• Protein ID: WP_029739941.1
• Coordinates: 193011..193277 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIJ47_RS01005 (KIJ47_01005)	188195..190039	+	1845	WP_038417274.1	cell envelope integrity protein TolA	-
KIJ47_RS01010 (KIJ47_01010)	190104..190562	+	459	WP_155268072.1	hypothetical protein	-
KIJ47_RS01015 (KIJ47_01015)	190585..190836	-	252	WP_071841167.1	hypothetical protein	-
KIJ47_RS01020 (KIJ47_01020)	191080..191358	+	279	WP_038417276.1	hypothetical protein	-
KIJ47_RS01025 (KIJ47_01025)	191388..192566	-	1179	WP_038417279.1	tyrosine-type recombinase/integrase	-
KIJ47_RS01030 (KIJ47_01030)	193011..193277	+	267	WP_029739941.1	DUF1778 domain-containing protein	Antitoxin
KIJ47_RS01035 (KIJ47_01035)	193265..193750	+	486	WP_029739942.1	GNAT family N-acetyltransferase	Toxin
KIJ47_RS01040 (KIJ47_01040)	194160..196070	+	1911	WP_029739943.1	NACHT domain-containing protein	-
KIJ47_RS01045 (KIJ47_01045)	196241..196539	+	299	Protein_205	integrase	-
KIJ47_RS01050 (KIJ47_01050)	196694..197764	-	1071	WP_029739945.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	179205..196489	17284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (54-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17681.39 Da
• Isoelectric Point: 9.0539

>T202480 WP_029739942.1 NZ_CP074584:193265-193750 [Enterobacter asburiae]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKTDTKQVAGFYSLATGSVNQVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLEADLLHDAVLRCYRVAENIGVRAIMVYALTEEAKSFYLHHGFKASQIQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T202480 NZ_CP074584:193265-193750 [Enterobacter asburiae]
GTGGGACGTGTAACAGCACCAGAACCGTTATCCAGTTCTCATCAACTTGCTGAGTTTGTCAGTGGTGAGGCTGTACTTGA
TGACTGGCTTAAACAGAGAGGATTAAAGAATCAGGCTCTCGGTGCCGCCCGAACGTTCGTTGTTTGTAAAACAGACACGA
AACAGGTTGCCGGTTTTTACTCTCTGGCCACGGGCAGCGTTAACCAAGTGGAGGCAACGGGAAGCCTCAGGCGCAATATG
CCAGATCCCATCCCCGTAATCATTCTTGCTCGCCTGGCTGTCGATGTTTCATTCCGTGGAAAAGGGCTCGAGGCTGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTTCGTGCGATTATGGTTTATGCACTGACTG
AAGAAGCCAAAAGCTTTTACCTTCACCATGGCTTTAAGGCATCGCAAATCCAGGAGCGCACATTGTTTCTCAAATTACCA
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10038.42 Da
• Isoelectric Point: 4.4733

>AT202480 WP_029739941.1 NZ_CP074584:193011-193277 [Enterobacter asburiae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVTDDATIGKLL
GRKPQWDV

Nucleotide

• Length: 267 bp

>AT202480 NZ_CP074584:193011-193277 [Enterobacter asburiae]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAGGAATCTCAAAGAGCACTCATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACAGACTTCATCCTGGAAATGGCATGTCAGGCTGCTGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCAGTTACGGATGACGCTACCATTGGTAAACTCCTG
GGAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A157GCY0