Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2848931..2849556 Replicon chromosome
Accession NZ_CP074580
Organism Klebsiella pneumoniae strain B30155

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KH752_RS13615 Protein ID WP_002882817.1
Coordinates 2848931..2849314 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KH752_RS13620 Protein ID WP_004150355.1
Coordinates 2849314..2849556 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH752_RS13600 (KH752_13600) 2846297..2847199 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KH752_RS13605 (KH752_13605) 2847196..2847831 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KH752_RS13610 (KH752_13610) 2847828..2848757 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KH752_RS13615 (KH752_13615) 2848931..2849314 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KH752_RS13620 (KH752_13620) 2849314..2849556 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KH752_RS13625 (KH752_13625) 2849761..2850678 + 918 WP_004895173.1 alpha/beta hydrolase -
KH752_RS13630 (KH752_13630) 2850692..2851633 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KH752_RS13635 (KH752_13635) 2851678..2852115 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KH752_RS13640 (KH752_13640) 2852112..2852972 - 861 WP_004146232.1 virulence factor BrkB family protein -
KH752_RS13645 (KH752_13645) 2852966..2853565 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202464 WP_002882817.1 NZ_CP074580:c2849314-2848931 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202464 NZ_CP099413:328118-328486 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202464 WP_004150355.1 NZ_CP074580:c2849556-2849314 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202464 NZ_CP099413:327900-328121 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References