Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2251948..2252758 Replicon chromosome
Accession NZ_CP074580
Organism Klebsiella pneumoniae strain B30155

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag KH752_RS10790 Protein ID WP_004178461.1
Coordinates 2251948..2252481 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KH752_RS10795 Protein ID WP_002887278.1
Coordinates 2252492..2252758 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KH752_RS10785 (KH752_10785) 2250779..2251900 + 1122 WP_002887282.1 cupin domain-containing protein -
KH752_RS10790 (KH752_10790) 2251948..2252481 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
KH752_RS10795 (KH752_10795) 2252492..2252758 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KH752_RS10800 (KH752_10800) 2252861..2254294 - 1434 WP_004894565.1 Cu(+)/Ag(+) sensor histidine kinase -
KH752_RS10805 (KH752_10805) 2254284..2254967 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KH752_RS10810 (KH752_10810) 2255139..2256524 + 1386 WP_004894567.1 efflux transporter outer membrane subunit -
KH752_RS10815 (KH752_10815) 2256542..2256886 + 345 WP_134920081.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T202462 WP_004178461.1 NZ_CP074580:c2252481-2251948 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202462 NZ_CP099402:c687453-687160 [Rhizobium sp. Pop5]
GTGAAGCTTACCTGGTCTGCGTTCGCGTTATCGGATCGCGACGCCATCTTCACATACATAGAAGGAGAAAATCCGTCAGC
CGCCATTCTGGTCGACGAACGGATCGTTGCTGCCGTCCGTCGCTTGATAGATTTTCCAGCGAGCGGCCGTGTTGGCAGAA
TCGCTGGCACCCGAGAACTGGTGATCAACGGCACGCCCTACGTCGCGGCGTATTCATTTAATGAAACAGCGGTCCGGATT
CTTCGCGTTCTCCATGGTGCGCAGGAATGGCCAGATACTTTGCCAACGGGCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202462 WP_002887278.1 NZ_CP074580:c2252758-2252492 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202462 NZ_CP099402:c687758-687450 [Rhizobium sp. Pop5]
ATGGCTGCAAATGCACTGGTACAAACTCGTATTGACGCCGAGATTCGGGATCGTGCCTCCGCAGTGCTTGGAAATATGGG
GCTTACGGTATCGGACGCGGTTCGTATCTTGCTCACCCGGACAGCCAACGAGGGTGCGCTGCCACTGGAGCTTCTTTCAG
GCAGCGAGGCCCATGACGCTTGGTTTCGCGCCAAGGTACTTGAAGCGCTGAACGATACGCGACCGGACGTTTCGGACGAT
GAAGTCGAGACACATTTTGCTGAACGTAGAGCGGCCGCCCGCCTTAACGCGGGCGCGCGCAAATCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References