Detailed information of TA system TA202458

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE-Phd
• Location: 16236..16847
• Replicon: plasmid p_unamed1
• Accession: NZ_CP074579
• Organism: Pseudomonas syringae strain Susan2139

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: KIJ28_RS25830
• Protein ID: WP_213632232.1
• Coordinates: 16497..16847 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: Q6VE98
• Locus tag: KIJ28_RS25825
• Protein ID: WP_011152898.1
• Coordinates: 16236..16484 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIJ28_RS25810 (KIJ28_25810)	13285..13980	-	696	WP_213632228.1	hypothetical protein	-
KIJ28_RS25815 (KIJ28_25815)	13981..14847	-	867	WP_249731738.1	HAD family hydrolase	-
KIJ28_RS25820 (KIJ28_25820)	14946..15905	-	960	WP_213632230.1	tyrosine-type recombinase/integrase	-
KIJ28_RS25825 (KIJ28_25825)	16236..16484	+	249	WP_011152898.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
KIJ28_RS25830 (KIJ28_25830)	16497..16847	+	351	WP_213632232.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIJ28_RS25835 (KIJ28_25835)	17350..17631	-	282	WP_057432554.1	hypothetical protein	-
KIJ28_RS25840 (KIJ28_25840)	17642..20464	-	2823	WP_249731739.1	RHS repeat-associated core domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..40409	40409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-101)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12895.70 Da
• Isoelectric Point: 4.4192

>T202458 WP_213632232.1 NZ_CP074579:16497-16847 [Pseudomonas syringae]
MNTFALRFTEVAQQSIEDQVEHLAITQGFSSAAQRIDSLIDAIQDKLLSTPLGYPVSPQLSELGVLHYRELNTDGYRVFY
EVMDSDGITGIVVLLVLGGKQSVEQALIRYCLHQPI

Nucleotide

• Length: 351 bp

>T202458 NZ_CP099401:c155924-155670 [Rhizobium sp. Pop5]
ATGAACAGCAAGCAGAGAAAGACCCTTGCGGCCGTATTCAAGGACCCGGTCTCGGGAACCATTGAATGGGCCGCTATAGA
GAATCTGCTTGCGGCTGCCGGAGCCGACGTCATTGAGGGGAACGGTTCCCGCGTTAAGTTTGAAAAAGACGGGATCATTG
CGAGCTTCCATCGACCTCATCCGGACAAGGAAGCCAAGCGTTATCAGGTGCGTGATGCTCGCGAGTTCCTGATGCAGATT
GGAGTAACGCCATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9329.71 Da
• Isoelectric Point: 9.8452

>AT202458 WP_011152898.1 NZ_CP074579:16236-16484 [Pseudomonas syringae]
MRVETISYLKRNAADLPLDEPLVVTQNGVPAYVVESYADRKRRDEAIALVKLLAISSREYAQGKHCSTDELKARLSRRFA
HK

Nucleotide

• Length: 249 bp

>AT202458 NZ_CP099401:c155673-155317 [Rhizobium sp. Pop5]
ATGAACACCATGACCTACAACGGGTATCACGCTCGCATCGAATTCGACGCTGAGGACGATGTGTTTTTCGGCAAGATTGC
AGGTATCTCCGATATAATCGGCTTTCACGGTGACAGTGTCGCTGAACTCAAGAAAGCCTTCCATGAGGCAGTCGATGACT
ACCTCGAGACCTGCAGGAGAATCGGCAAAGAGCCTCAGCGACCCTATTCTGGGAAGATGATGTTTCGTGTCGCTCCCGAA
GTCCATCGCCGAGCAGCTCTTGCTGCTGAGCTTTCGGGAAAGAGTCTGAACCAGTGGGCAGAAGAGGTGCTGGAAGAAGC
CGCCGACCATTTTGCCGAGGCGCGCCTGCCGGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7V8LY57