Detailed information of TA system TA202457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 5428382..5428901
• Replicon: chromosome
• Accession: NZ_CP074578
• Organism: Pseudomonas syringae strain Susan2139

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7Z6UJD9
• Locus tag: KIJ28_RS24080
• Protein ID: WP_010434526.1
• Coordinates: 5428382..5428672 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S6UWW5
• Locus tag: KIJ28_RS24085
• Protein ID: WP_002551444.1
• Coordinates: 5428662..5428901 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIJ28_RS24045 (KIJ28_24045)	5424144..5424356	+	213	WP_236523103.1	transposase	-
KIJ28_RS24050 (KIJ28_24050)	5424773..5424991	+	219	WP_236444532.1	addiction module killer	-
KIJ28_RS24055 (KIJ28_24055)	5424967..5425332	+	366	WP_058444494.1	putative addiction module antidote protein	-
KIJ28_RS24060 (KIJ28_24060)	5425425..5426069	-	645	WP_213631348.1	histidine phosphatase family protein	-
KIJ28_RS24065 (KIJ28_24065)	5426169..5426510	-	342	WP_213631349.1	DUF5713 family protein	-
KIJ28_RS24070 (KIJ28_24070)	5426698..5427120	+	423	WP_122591334.1	DUF1493 family protein	-
KIJ28_RS24075 (KIJ28_24075)	5427427..5428164	+	738	WP_074595064.1	hypothetical protein	-
KIJ28_RS26165	5428175..5428306	-	132	Protein_4756	GNAT family N-acetyltransferase	-
KIJ28_RS24080 (KIJ28_24080)	5428382..5428672	-	291	WP_010434526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KIJ28_RS24085 (KIJ28_24085)	5428662..5428901	-	240	WP_002551444.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KIJ28_RS24090 (KIJ28_24090)	5429065..5429538	-	474	WP_213631350.1	GNAT family N-acetyltransferase	-
KIJ28_RS24095 (KIJ28_24095)	5429621..5430190	-	570	WP_213631351.1	GNAT family N-acetyltransferase	-
KIJ28_RS24100 (KIJ28_24100)	5430315..5430845	+	531	WP_072367094.1	hypothetical protein	-
KIJ28_RS24105 (KIJ28_24105)	5430883..5432157	-	1275	WP_213631352.1	OprD family porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-64)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11187.03 Da
• Isoelectric Point: 10.0984

>T202457 WP_010434526.1 NZ_CP074578:c5428672-5428382 [Pseudomonas syringae]
MTYNLEFDARALKEWHKLGDTVRQQLKKKLATILVAPRVEANRLHALPDCYKIKLRSSGYRLVYQVIDQEVVVFVVAVDK
REREEVYRKAADRLGG

Nucleotide

• Length: 291 bp

>T202457 NZ_CP099401:135042-135335 [Rhizobium sp. Pop5]
GTGAAGCTCACCTGGTCTGCGTTCGCGTTATCGGATCGCGACGCCATCTTCACATACATAGAAGCAGAAAATCCGGCCGC
CGCCATTCTGGTCGACGAACGGATCGCTGCTGCCGTCCGTCGGTTGATAGATTTTCCAGCGAGCGGCCGTGTTGGCAGAA
TCGCTGGCACCCGAGAACTGGTGATCAACGGCACGCCCTACGTCGCGGCGTATTCATTTAATGAAACAGCGGTCCGAATT
CTTCGCGTTCTCCACGGTGCGCAGGAATGGCCAGATACTTTGCCAACGGGATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8955.34 Da
• Isoelectric Point: 4.6017

>AT202457 WP_002551444.1 NZ_CP074578:c5428901-5428662 [Pseudomonas syringae]
MASINVRIDDDLKARAYLELEKLGVTPSELLRQTLQYVAERGQLPFKTVLMTEEDEALLATVRERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT202457 NZ_CP099401:134737-135045 [Rhizobium sp. Pop5]
ATGGCTGCAAATGCACTGGTACAAACTCGTATTGACGCCGAGATTCGGGATCGTGCCTCCGCAGTGCTTGGAAATATGGG
GCTTACGGTATCGGACGCGGTTCGTATCTTGCTCACCCGGACAGCCAACGAGGGTGCGCTGCCGCTGGAGCTTCTTTCAG
GCAGCGAGGCCCATGACGCTTGGTTTCGCGCCAAGGTACTTGAAGCGCTGAACGATACGCGACCGGACGTTTCGGACGAT
GAAGTCGAGACCCATTTTGCTGAACGTAGAGCGGCCGCTCGCCTTAACGCGGGCGCGCGCAAATCGTGA

Structures

Toxin

• AlphaFold DB: A0A7Z6UJD9

Antitoxin

• AlphaFold DB: A0A0K8M1G9