202455

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 968681..969315 Replicon chromosome
Accession NZ_CP074578
Organism Pseudomonas syringae strain Susan2139

Toxin (Protein)


Gene name vapC Uniprot ID A0A193SM35
Locus tag KIJ28_RS04320 Protein ID WP_003345997.1
Coordinates 968681..969085 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3M4XXE1
Locus tag KIJ28_RS04325 Protein ID WP_003345999.1
Coordinates 969085..969315 (-) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIJ28_RS04290 (KIJ28_04290) 964465..964947 + 483 WP_003345989.1 PAS domain-containing protein -
KIJ28_RS04295 (KIJ28_04295) 965044..966300 + 1257 WP_004402530.1 3-phosphoshikimate 1-carboxyvinyltransferase -
KIJ28_RS04300 (KIJ28_04300) 966323..966985 - 663 WP_003408532.1 ChrR family anti-sigma-E factor -
KIJ28_RS04305 (KIJ28_04305) 966985..967497 - 513 WP_213632060.1 sigma-70 family RNA polymerase sigma factor -
KIJ28_RS04310 (KIJ28_04310) 967733..967921 - 189 WP_024663313.1 hypothetical protein -
KIJ28_RS04315 (KIJ28_04315) 968273..968494 + 222 WP_003345996.1 hypothetical protein -
KIJ28_RS04320 (KIJ28_04320) 968681..969085 - 405 WP_003345997.1 tRNA(fMet)-specific endonuclease VapC Toxin
KIJ28_RS04325 (KIJ28_04325) 969085..969315 - 231 WP_003345999.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KIJ28_RS04330 (KIJ28_04330) 969432..970313 - 882 WP_213631580.1 aldose 1-epimerase -
KIJ28_RS04335 (KIJ28_04335) 970288..971259 - 972 WP_213631581.1 DMT family transporter -
KIJ28_RS04340 (KIJ28_04340) 971344..972624 - 1281 WP_003421655.1 TRAP transporter large permease -
KIJ28_RS04345 (KIJ28_04345) 972625..973152 - 528 WP_003408544.1 TRAP transporter small permease -
KIJ28_RS04350 (KIJ28_04350) 973216..974241 - 1026 WP_010424887.1 TRAP transporter substrate-binding protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-125)

Antitoxin

(7-34)


Sequences


Toxin        


Download         Length: 135 a.a.        Molecular weight: 14946.21 Da        Isoelectric Point: 6.8620

>T202455 WP_003345997.1 NZ_CP074578:c969085-968681 [Pseudomonas syringae]
MLKYMLDTNICIFTIKNKPTSVREAFNLHHGQLCISAITLMELVYGAEKSLSPERNLAVVEGFTARLEVLPYDSDAAAHT
GMIRAELARSGTPIGPYDQMIAGHARSLGLVVITNNQREFRRVEGLRVEDWVSQ

Download         Length: 405 bp

>T202455 NZ_CP099397:4253345-4253764 [Pseudomonas hydrolytica]
ATGATCCTGCTTGATACCAACGTGCTGTCGGAACTCATGCGTGCCAAGCCTGCGCCTCAGGTTCTGGAGTGGGTTGACGC
TCAGCCTGTCGGGGATCTGGTAATCACCTCGATCACCGTTGCGGAAATTCTCTATGGCATTGCCAGGATGCCGGACGGGA
AACGCAAGCAGGGACTGCTCGATGTAGCCTCGGTCATGTTCGATGAAGACTTCGCGGGCAATATCCTGCCGTTCGATGCA
GATGCCGCTGTGCATTACGCAGAGATAGCCGCAGAAACCGAGGCTAAAGGGCGGGTGGTTGATATGGCCGATGCGCAGAT
CGCTGCAATCGGTCGGCTACATGATGCCGTGATCGCAACTCGCAATATTCGCCACTTCGAAACGCTAGGCGTTGCCCTGG
TAGATCCCTGGAGCAATTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8580.59 Da        Isoelectric Point: 4.5557

>AT202455 WP_003345999.1 NZ_CP074578:c969315-969085 [Pseudomonas syringae]
MEQSTVFKSNRSQAVRLPKAVALPDDVKRVDVVAVGRTRIISPAGEMWNSWFDGESVSDDFMAEREQPAEQQRESL

Download         Length: 231 bp

>AT202455 NZ_CP099397:4253103-4253348 [Pseudomonas hydrolytica]
ATGGCCGCTATCACTATTCGAAACCTCGACGACGACTTGAAAGCCCAGCTGCGTATTGTCGCGGCTAGCCACGGTCGCTC
CATGGAAGAAGAGGTCCGGTTCATCATTCGGCAGGCTCTGTCACGTCAAGAAAAGCGTGGCGGCTTGGGCAGTCGTATTC
ACTCCCGCTTTGCCGCTGTCGGTGGGGCTGACCTTGAGCTGCCAGAACGCAAAACCAAAGCCCGAGCCGCGAGCTTTGAT
GAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A193SM35


Antitoxin

Source ID Structure
AlphaFold DB A0A3M4XXE1

References