Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-YafN |
| Location | 174278..174813 | Replicon | plasmid pCS18F-NDM-Fos |
| Accession | NZ_CP074577 | ||
| Organism | Escherichia coli strain CS18F | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | V0VJJ7 |
| Locus tag | KHZ70_RS24640 | Protein ID | WP_000222760.1 |
| Coordinates | 174278..174565 (-) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | V0VIP2 |
| Locus tag | KHZ70_RS24645 | Protein ID | WP_001132900.1 |
| Coordinates | 174562..174813 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KHZ70_RS24615 (169861) | 169861..171082 | - | 1222 | Protein_190 | ISL3 family transposase | - |
| KHZ70_RS24620 (171182) | 171182..171546 | + | 365 | Protein_191 | IS1 family transposase | - |
| KHZ70_RS24625 (171601) | 171601..172383 | - | 783 | WP_001442137.1 | IS21-like element IS100kyp family helper ATPase IstB | - |
| KHZ70_RS24630 (172380) | 172380..173402 | - | 1023 | WP_000255956.1 | IS21-like element IS100 family transposase | - |
| KHZ70_RS24635 (173491) | 173491..173838 | + | 348 | Protein_194 | IS1-like element IS1A family transposase | - |
| KHZ70_RS25075 (173854) | 173854..174174 | - | 321 | Protein_195 | serine acetyltransferase | - |
| KHZ70_RS24640 (174278) | 174278..174565 | - | 288 | WP_000222760.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KHZ70_RS24645 (174562) | 174562..174813 | - | 252 | WP_001132900.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| KHZ70_RS24650 (175780) | 175780..176637 | - | 858 | WP_000774297.1 | incFII family plasmid replication initiator RepA | - |
| KHZ70_RS24655 (176630) | 176630..177112 | - | 483 | WP_001273588.1 | hypothetical protein | - |
| KHZ70_RS25080 (177105) | 177105..177152 | - | 48 | WP_229471593.1 | hypothetical protein | - |
| KHZ70_RS25085 (177143) | 177143..177394 | + | 252 | WP_223195197.1 | replication protein RepA | - |
| KHZ70_RS24665 (177411) | 177411..177668 | - | 258 | WP_000083845.1 | replication regulatory protein RepA | - |
| KHZ70_RS24670 (177952) | 177952..178101 | - | 150 | WP_001312851.1 | Hok/Gef family protein | - |
| - (178145) | 178145..178206 | + | 62 | NuclAT_1 | - | - |
| - (178145) | 178145..178206 | + | 62 | NuclAT_1 | - | - |
| - (178145) | 178145..178206 | + | 62 | NuclAT_1 | - | - |
| - (178145) | 178145..178206 | + | 62 | NuclAT_1 | - | - |
| KHZ70_RS24675 (178462) | 178462..178536 | - | 75 | Protein_204 | endonuclease | - |
| KHZ70_RS24680 (178782) | 178782..178994 | - | 213 | WP_001299730.1 | ANR family transcriptional regulator | - |
| KHZ70_RS24685 (179130) | 179130..179690 | - | 561 | WP_032072881.1 | fertility inhibition protein FinO | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | sitABCD / mph(A) / aph(3')-Ia / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / fosA3 | iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN | 1..189141 | 189141 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11105.15 Da Isoelectric Point: 10.5832
>T202450 WP_000222760.1 NZ_CP074577:c174565-174278 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR
Download Length: 288 bp
>T202450 NZ_CP099396:c2503170-2502823 [Lacticaseibacillus rhamnosus]
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG
ATGACATATAAGGCTAAGCAAGGTGATGTGGTGTGGCTTGATTTTGATCCGTCCGAGGGTCATGAAATTAGAAAAAGATG
CCCAGCTGTTGTTTTAAGCAGTAACGCATACAATCGTGCAACCGGATTTGTGATTGTCGCACCAATTACCTCAACAATTC
GGACACTACCTGGTTATGTTGATATTGTATCCAATAAAATTCGTGGTCAGGTTGTCACTTCGCAGGTTTATTCTCTGGAT
GTCACTGAACAAGGCAACCGAAAAATCGATTTTATTGAAAGAATGAACATTGAAGATTTTTATACAGTCGCTCAATTCAC
CAAAATGAATTTTGATTTTAAATTTTAG
Antitoxin
Download Length: 84 a.a. Molecular weight: 9211.41 Da Isoelectric Point: 4.0780
>AT202450 WP_001132900.1 NZ_CP074577:c174813-174562 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL
Download Length: 252 bp
>AT202450 NZ_CP099396:c2503388-2503170 [Lacticaseibacillus rhamnosus]
ATGATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGA
GGCGTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAAC
CATTATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA
ATGATGGATGTGAAAGTTGTAAAACGTGGAAACTCGTTAGCTTTATCACTTCCGAGCAGTGCGGGTTTCAAACGAGGCGA
GGCGTTTTTGCTGATTTCAGATGAAAAAGGTGGTTATCTGTTGATCCCAAAGGTAAAAAATCCCTATACCAAAGCAAAAC
CATTATCATTCCATCAAGAAGAAGCTTGGCCGGATTTTGACTATGAGGATATTGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|