Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 60807..61450 Replicon plasmid pCS18F-NDM-Fos
Accession NZ_CP074577
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KHZ70_RS24085 Protein ID WP_001034044.1
Coordinates 61034..61450 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KHZ70_RS24080 Protein ID WP_001261286.1
Coordinates 60807..61037 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS24060 (56369) 56369..57058 - 690 WP_001513524.1 RES family NAD+ phosphorylase -
KHZ70_RS24065 (57477) 57477..57707 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin -
KHZ70_RS24070 (57704) 57704..58120 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin -
KHZ70_RS24075 (58282) 58282..60420 - 2139 WP_001513523.1 AAA family ATPase -
KHZ70_RS24080 (60807) 60807..61037 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHZ70_RS24085 (61034) 61034..61450 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KHZ70_RS24090 (61525) 61525..63090 + 1566 WP_001128474.1 AAA family ATPase -
KHZ70_RS24095 (63075) 63075..64097 + 1023 WP_000361402.1 helicase UvrD -
KHZ70_RS24100 (64351) 64351..65047 - 697 Protein_82 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / mph(A) / aph(3')-Ia / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / fosA3 iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN 1..189141 189141
- flank IS/Tn - - 64351..64863 512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T202449 WP_001034044.1 NZ_CP074577:61034-61450 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T202449 NZ_CP099396:c521675-521490 [Lacticaseibacillus rhamnosus]
TTGCAGCGTCAAGGTCATGTAAAATTAGCTTCTCTACATGACCATGCTTTAAAGGGTGCTCACAGCGGCGAACGAGCATT
GCGTGTTGCTCCTGATTGGCTTCTCGTTTATAAAGTCGATGCTGAAGCGTTAATTTTAATGCTTCTTGCAACAGGCACAC
ACCGCGATACATTGAACATTGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT202449 WP_001261286.1 NZ_CP074577:60807-61037 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT202449 NZ_CP099396:c521975-521703 [Lacticaseibacillus rhamnosus]
ATGACCAAAAAATCCCGCATCAGCGTTAGGATTGATACCAAAACTAAAGAACGAGCGCTCCATGTTCTTAATAGCATGGG
ATTAGATATTTCCTCGGCTATTAACATGTACCTGAAACGGATTGGTGACACCGGTGCATTGCCATTTACACCTCCAATGT
CATTTGTTGATCAGCTTCAAGTTGCAGAAGCTGATGTTAAAGCGGGGCGAATAAAAAGCTTCAAGACTGTCGATGCTTTG
ATGAAGGATTTATACAGTGATGTTGACGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References