Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 57477..58120 Replicon plasmid pCS18F-NDM-Fos
Accession NZ_CP074577
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag KHZ70_RS24070 Protein ID WP_001044768.1
Coordinates 57704..58120 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag KHZ70_RS24065 Protein ID WP_001261287.1
Coordinates 57477..57707 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS24035 (52911) 52911..53084 - 174 Protein_69 RepB family plasmid replication initiator protein -
KHZ70_RS24040 (53784) 53784..54566 - 783 WP_001513526.1 site-specific integrase -
KHZ70_RS24045 (54567) 54567..54872 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
KHZ70_RS24050 (54874) 54874..55092 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
KHZ70_RS24055 (55648) 55648..56337 + 690 WP_001513525.1 helix-turn-helix domain-containing protein -
KHZ70_RS24060 (56369) 56369..57058 - 690 WP_001513524.1 RES family NAD+ phosphorylase -
KHZ70_RS24065 (57477) 57477..57707 + 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHZ70_RS24070 (57704) 57704..58120 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
KHZ70_RS24075 (58282) 58282..60420 - 2139 WP_001513523.1 AAA family ATPase -
KHZ70_RS24080 (60807) 60807..61037 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KHZ70_RS24085 (61034) 61034..61450 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KHZ70_RS24090 (61525) 61525..63090 + 1566 WP_001128474.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / mph(A) / aph(3')-Ia / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / fosA3 iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN 1..189141 189141


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T202448 WP_001044768.1 NZ_CP074577:57704-58120 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T202448 NZ_CP099395:51327-52232 [Paraburkholderia bryophila]
ATGTCCGCAGTCCCGACCGCAACCGCCGATCAGCATGCCTCGCTCGAAGCACGCGCCGCGCGCTTTCCGGCGCAGCAGAT
CTGGCTCGCTTCGCAACGCGCGGTAAGTTATCGCGAAGCGGAGTGCGCGGACAGCGAGAGCAAGCGCAGCGCGTTGCCCC
TCGTGCTGTTGCACGGCATCGGTTCGGGCGCCGCCTCGTGGGTGCAGCAGTTTGAAGTGCTCGGCGCGACACGCCGTGTA
CTGGCCTGGGACGCGCCGGGTTATGGCGCGTCCACGCCGGTCGCGGCCGAATCGCCGAACGCCATGGATTACGCAACCGT
CCTGAACGGATGGCTCGACGCCCTCGGCATTGAACGCTGTGTGCTGCTCGGTCATTCGCTGGGCGCGATCATCGCCGGTG
CGTTTGCGCTTGCCTATCCGCAGCGTGTGGCCGGCCTGCTGCTGTTGTCGCCCGCCGGCGGTTACGGCGCGGCTTCCGCC
GAGGTACGCAACACAAAACGCGATCAGCGCCTCGCGATGCTGAACGAACTCGGCCCGCAAGGCCTCGCCGAACAACGCAG
CACCAACATGTTGTCCGCGCAAGCCAACGACAATGCCCGCGCCTGGGTGCGCTGGAACATGTCGCGCGTGATTCCGCACG
GCTACGCGCAGGCCACGCATCTGCTCGCCAACGCCGACCTTGCCGCCGACCTCGCGCGCTACACCGGCCGCATCGATGTC
GCCGTCGGCGCGGACGACACCATTACGCCCCCGGCCGCTTGCGAGCGACTCGCGCTAGCGGCGCGCACCTCACTGCAGGT
CGTGCCGCACGCGGGGCACGCCGGCTATATCGAAGCTCCCGCCGCGACCACCGCGATCATCGACACGTTCTGCCGCGTGA
GCGACGGACAACGGAGCCCTGAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT202448 WP_001261287.1 NZ_CP074577:57477-57707 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT202448 NZ_CP099395:52229-53113 [Paraburkholderia bryophila]
ATGACGCCCGACCTCAAAAACGCCGAGCGCGACGCGGACGACGACCGCAACGACACCGGTAGCGAAGCCAGCTACCGTGT
GCCGGGTCTCGAACGCGGTCTGAGGATCCTCACGGAATTCTCGCCACGCGAGCCGGTTCTCGGCGCACCGGAATTATCGA
AGCGCCTGAAGATTCCCCGCACGACGGTGTTCCGTCTGCTGCAAACGCTCGAATCGCTCGGCTTTCTCGAACGCGCCGAC
AAGGACCGCAACTATCGCCTCGGCGTCGCGGTGCTGCGGCTCGGTTTCGAATATCTGAGCTCGCTCGAATTGACCGACCT
CGGCCTGCCGATCATCGAAGCATTGCGCGACGACACCGGTTTGACGAGCCATATCGTGATTCGCGACGGACGCGATGTGG
TGTTCGTCGCCAAGGCACAAAGCCACGCGCCGATTTTCAGCTCGGTGAAGGTGAACGTCGGCACGCGTCTGCCGGCGTAC
GCAACGACACACGGCCAGGTGCTGATGGGCGACATGACGCTCGACGACCTGAAGAAGCTGTATCCCGAACCGGAACTCGA
GCGCTTCACGAAACAGACGCCGATCACGCTGGAAGACCTGTACGAACGGATTCGCGACGACGCCAAGCGTGGCTTCGCGA
TCAGCGAGTCGTCGTTCGAACGCGGTATTTCGGTGGTCAGCGCGCCGGTGCGCAACGACACGGGCCGCATCGTCGCCGTG
ATCACGACGACGATTCCGCGCCACGAGATCGACGCGTCGCTGCTGGACAGCGGACTGATCGACAAGGTGCGCCGCGCAGC
CGAAGAACTCTCGCAGCGGCTCAACTACCGGCCGAAGAGCGGGCCGAATGCGGGCAGCAAATACATGAAGGCACTGGGGC
TCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References