Detailed information of TA system TA202447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 54567..55092
• Replicon: plasmid pCS18F-NDM-Fos
• Accession: NZ_CP074577
• Organism: Escherichia coli strain CS18F

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: KHZ70_RS24045
• Protein ID: WP_001159871.1
• Coordinates: 54567..54872 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: H9TJP1
• Locus tag: KHZ70_RS24050
• Protein ID: WP_000813630.1
• Coordinates: 54874..55092 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHZ70_RS24015 (49775)	49775..49987	-	213	WP_001523378.1	hypothetical protein	-
KHZ70_RS24020 (50716)	50716..50973	+	258	WP_015387373.1	hypothetical protein	-
KHZ70_RS24025 (51069)	51069..51515	-	447	WP_053270998.1	hypothetical protein	-
KHZ70_RS24030 (51680)	51680..52908	+	1229	WP_148718718.1	IS3-like element IS2 family transposase	-
KHZ70_RS24035 (52911)	52911..53084	-	174	Protein_69	RepB family plasmid replication initiator protein	-
KHZ70_RS24040 (53784)	53784..54566	-	783	WP_001513526.1	site-specific integrase	-
KHZ70_RS24045 (54567)	54567..54872	-	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
KHZ70_RS24050 (54874)	54874..55092	-	219	WP_000813630.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KHZ70_RS24055 (55648)	55648..56337	+	690	WP_001513525.1	helix-turn-helix domain-containing protein	-
KHZ70_RS24060 (56369)	56369..57058	-	690	WP_001513524.1	RES family NAD+ phosphorylase	-
KHZ70_RS24065 (57477)	57477..57707	+	231	WP_001261287.1	type II toxin-antitoxin system VapB family antitoxin	-
KHZ70_RS24070 (57704)	57704..58120	+	417	WP_001044768.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / mph(A) / aph(3')-Ia / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / fosA3	iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroE / iroN	1..189141	189141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T202447 WP_001159871.1 NZ_CP074577:c54872-54567 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T202447 NZ_CP099394:4455487-4455669 [Paraburkholderia bryophila]
ATGAACAGCGCCGCGGTAATGAAATGGATTCAGGCGAATGACTGGCAGTTGGTCCGTATCTCGGGCAGCCACCATCATTT
CCGGCATCCGTTCAGGGCGGGTCTCGTGACGATCCCTCACCCCAAGAAAGATCTGCCGCCCGGCACATTGAACAGCATCC
TGAAACAGGCGGGCCTGAAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8354.27 Da
• Isoelectric Point: 4.5203

>AT202447 WP_000813630.1 NZ_CP074577:c55092-54874 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT202447 NZ_CP099394:4455666-4456073 [Paraburkholderia bryophila]
ATGAAAAATCTGATTTTCCCGATCGCGCTCGAATCGGGCGACGACCAGCATGCGTATGGCGTGGTGGTGCCGGACCTTCC
CGGCTGCTTTGCGGCCGGCGACACGCTTGAAGAGGCTTTTGCCAACGCGAAGCTGGCGGTCGAGTCGCATCTCGACACGC
TGCTCGACGAAGGGCTACCGGTGCCGCAGCCGCTCAAGCTGAGCGAGCATCGGCGCAATCCGGATTACGCGGGGTTTATC
TGGGGATTCGTCGTCACCCGGAATATTCCGGCTCTGAAGAAGGCCGTGCGCATCAACATCTCGCTGCCCGAGGTGCTGGT
CCAGGATATCGACATTTACGCGCAAACTCGCGGCTTGTCGCGGTCGTCGTTTCTCGCGCTGGCCGCGGAGCATGAGATGG
CGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A829CEF5