Detailed information of TA system TA202442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4709887..4710518
• Replicon: chromosome
• Accession: NZ_CP074576
• Organism: Escherichia coli strain CS18F

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: KHZ70_RS22670
• Protein ID: WP_001260301.1
• Coordinates: 4709887..4710162 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: KHZ70_RS22675
• Protein ID: WP_000593557.1
• Coordinates: 4710159..4710518 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHZ70_RS22655 (4704891)	4704891..4705958	+	1068	WP_000361470.1	HlyD family secretion protein	-
KHZ70_RS22660 (4705955)	4705955..4708690	+	2736	WP_000149178.1	ribosome-associated ATPase/putative transporter RbbA	-
KHZ70_RS22665 (4708690)	4708690..4709814	+	1125	WP_001318088.1	ABC transporter permease	-
KHZ70_RS22670 (4709887)	4709887..4710162	+	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
KHZ70_RS22675 (4710159)	4710159..4710518	+	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KHZ70_RS22680 (4710638)	4710638..4711039	-	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
KHZ70_RS22685 (4711045)	4711045..4711851	-	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
KHZ70_RS22690 (4711848)	4711848..4712612	-	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
KHZ70_RS22695 (4712612)	4712612..4713445	-	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
KHZ70_RS22700 (4713442)	4713442..4714386	-	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T202442 WP_001260301.1 NZ_CP074576:4709887-4710162 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T202442 NZ_CP099390:c4967822-4967448 [Citrobacter braakii]
ATGGTCACAGGATCTGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGAGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATCGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT202442 WP_000593557.1 NZ_CP074576:4710159-4710518 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT202442 NZ_CP099390:c4968064-4967822 [Citrobacter braakii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCCGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1