Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3944973..3945470 Replicon chromosome
Accession NZ_CP074576
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KHZ70_RS19180 Protein ID WP_001173343.1
Coordinates 3944973..3945146 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag KHZ70_RS19185 Protein ID WP_001317561.1
Coordinates 3945174..3945470 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS19170 (3941643) 3941643..3943100 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
KHZ70_RS19175 (3943337) 3943337..3944854 + 1518 WP_000003805.1 lysine--tRNA ligase -
KHZ70_RS19180 (3944973) 3944973..3945146 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KHZ70_RS19185 (3945174) 3945174..3945470 - 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KHZ70_RS19190 (3945697) 3945697..3945969 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KHZ70_RS19195 (3945981) 3945981..3946211 - 231 WP_000371717.1 4Fe-4S mono-cluster protein YjdI -
KHZ70_RS19200 (3946391) 3946391..3948022 + 1632 WP_001216477.1 sensor histidine kinase -
KHZ70_RS19205 (3948019) 3948019..3948738 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T202437 WP_001173343.1 NZ_CP074576:c3945146-3944973 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T202437 NZ_CP099390:865526-865933 [Citrobacter braakii]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGCTGCTCATTCATGGGCTGGTGGCGGT
ATTTATATTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TCCGTAGCCAGCGCCGAATCAATGCCTGTCAGGGCGAGATTCGGCTGTTGATGGACGGTCGTCTACGCTGGCAAGGGCAA
GAGTGGACTATTGCTAGCGCACCCTGGATGGTCAAAACAGGCATGATGCTGCGTTTACGTTCCGATGCCGGAAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGACGCGGAGTGGCGGGATTTGCGGCGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT202437 WP_001317561.1 NZ_CP074576:c3945470-3945174 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT202437 NZ_CP099390:865279-865545 [Citrobacter braakii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGATGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGCTCAGCTAGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References