Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3403343..3404141 Replicon chromosome
Accession NZ_CP074576
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name yeeV Uniprot ID Q8VRA6
Locus tag KHZ70_RS16570 Protein ID WP_000854730.1
Coordinates 3403764..3404141 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0P0SQV8
Locus tag KHZ70_RS16565 Protein ID WP_001285481.1
Coordinates 3403343..3403717 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS16540 (3399163) 3399163..3399840 - 678 Protein_3262 IS66-like element ISEc22 family transposase -
KHZ70_RS16545 (3400036) 3400036..3401581 + 1546 Protein_3263 IS21-like element ISEc12 family transposase -
KHZ70_RS16550 (3401596) 3401596..3402342 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
KHZ70_RS16555 (3402436) 3402436..3402630 + 195 WP_001334544.1 DUF987 domain-containing protein -
KHZ70_RS16560 (3402649) 3402649..3403293 + 645 WP_000086759.1 hypothetical protein -
KHZ70_RS16565 (3403343) 3403343..3403717 + 375 WP_001285481.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KHZ70_RS16570 (3403764) 3403764..3404141 + 378 WP_000854730.1 TA system toxin CbtA family protein Toxin
KHZ70_RS16575 (3404138) 3404138..3404630 + 493 Protein_3269 DUF5983 family protein -
KHZ70_RS16580 (3404709) 3404709..3405697 - 989 Protein_3270 IS630 family transposase -
KHZ70_RS16585 (3405845) 3405845..3407438 - 1594 Protein_3271 IS66 family transposase -
KHZ70_RS16590 (3407469) 3407469..3407819 - 351 WP_000624707.1 IS66 family insertion sequence element accessory protein TnpB -
KHZ70_RS16595 (3407816) 3407816..3408107 - 292 Protein_3273 transposase -
KHZ70_RS24950 (3408149) 3408149..3408343 + 195 WP_001313570.1 DUF957 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - pic 3370553..3412383 41830
- inside IScluster/Tn - - 3389353..3408107 18754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14212.21 Da        Isoelectric Point: 7.2923

>T202435 WP_000854730.1 NZ_CP074576:3403764-3404141 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRMVNDIIRGEHSEAKR

Download         Length: 378 bp

>T202435 NZ_CP099389:4775646-4775957 [Citrobacter braakii]
ATGCATGTTATCAGCCGAAAACCTTTCAACGAGGCAATACTCCGTTTTCCAAACCACATGGCAGCACTCGTTGATTTGCT
GAATATTTTAGAGAAAAAAATGTTCCACACTCCTGAAGAGATGAAGCAGTACATACCTTCACTGGATAATTTTAAATACA
GAAATAAATGGTGGGTGATTAATGTTTCAGGTAACTGCTTGAGACTTATTGCCTATATTGATTTCAAACTGCAAAAGGTA
TTTGTGAAGCATATCGTTCACCATGCCGAGTATGACAGACTAACCACATACTATCGGGGTCACAAAGAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13817.53 Da        Isoelectric Point: 4.7511

>AT202435 WP_001285481.1 NZ_CP074576:3403343-3403717 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQLELMLTIG
ELNPRHQHTVTLYARGLTCEADTLGSCGYVYMAVYPTLAPATTS

Download         Length: 375 bp

>AT202435 NZ_CP099389:4775954-4776406 [Citrobacter braakii]
ATGAGAACGCATGAAAGTCATCATATAGACACAGCCAGTGTAAAATTGATGATCGATACCTTTACCGATGCAGTGAAAAA
AATCCCCCTGTTAGGCAAAGAACAAAATGAGACAGAGTACCGAAAAGCACTCGCTTTGGTGGAGTTTCTGGTTGATCGTG
ACGACCTGGAAAACCCGTTGTTTGAGCTGCTTTCAGCTCGGATCCGCGACTACGAAAAACATGCGCCTGAGTTCAGTGCC
CTCAACCAACAACTGGAACATACTCCTCATGGCGTTGCGTTACTGCGTACGCTCATGGATCAATATGGTCTCAAAGCAGC
AGATCTTGCCAACGAACTTGGTTCAAAATCTAATGTCAGCAACATCTTGAATGGTCGCAGAGCACTGACTGTAAGTCATA
TTAAAATGCTCGCAGAACGCTTTAACCTTCCGGCAGACGCATTCATCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2V671

Antitoxin

Source ID Structure
AlphaFold DB A0A0P0SQV8

References