Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3122437..3123055 Replicon chromosome
Accession NZ_CP074576
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KHZ70_RS15225 Protein ID WP_001291435.1
Coordinates 3122837..3123055 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KHZ70_RS15220 Protein ID WP_000344800.1
Coordinates 3122437..3122811 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS15210 (3117526) 3117526..3118719 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHZ70_RS15215 (3118742) 3118742..3121891 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KHZ70_RS15220 (3122437) 3122437..3122811 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KHZ70_RS15225 (3122837) 3122837..3123055 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KHZ70_RS15230 (3123227) 3123227..3123778 + 552 WP_000102564.1 maltose O-acetyltransferase -
KHZ70_RS15235 (3123894) 3123894..3124364 + 471 WP_000136192.1 YlaC family protein -
KHZ70_RS15240 (3124528) 3124528..3126078 + 1551 WP_001317659.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KHZ70_RS15245 (3126120) 3126120..3126473 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KHZ70_RS15255 (3126852) 3126852..3127163 + 312 WP_000409911.1 MGMT family protein -
KHZ70_RS15260 (3127194) 3127194..3127766 - 573 WP_000779821.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202434 WP_001291435.1 NZ_CP074576:3122837-3123055 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T202434 NZ_CP099389:c4774185-4773811 [Citrobacter braakii]
ATGGTCACAGGATCTGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGCGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATCGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGTG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT202434 WP_000344800.1 NZ_CP074576:3122437-3122811 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT202434 NZ_CP099389:c4774427-4774185 [Citrobacter braakii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCCGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References