Detailed information of TA system TA202432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2704824..2705529
• Replicon: chromosome
• Accession: NZ_CP074576
• Organism: Escherichia coli strain CS18F

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: KHZ70_RS13070
• Protein ID: WP_000539521.1
• Coordinates: 2704824..2705210 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KHZ70_RS13075
• Protein ID: WP_001280945.1
• Coordinates: 2705200..2705529 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHZ70_RS13050 (2700828)	2700828..2701454	+	627	WP_001295292.1	glutathione S-transferase GstB	-
KHZ70_RS13055 (2701451)	2701451..2702566	-	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
KHZ70_RS13060 (2702677)	2702677..2703060	-	384	WP_000497137.1	biofilm formation regulator BssR	-
KHZ70_RS13065 (2703273)	2703273..2704598	+	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
KHZ70_RS13070 (2704824)	2704824..2705210	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KHZ70_RS13075 (2705200)	2705200..2705529	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
KHZ70_RS13080 (2705599)	2705599..2706927	-	1329	WP_000086919.1	GGDEF domain-containing protein	-
KHZ70_RS13085 (2706935)	2706935..2709283	-	2349	Protein_2580	EAL domain-containing protein	-
KHZ70_RS13090 (2709461)	2709461..2710372	-	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T202432 WP_000539521.1 NZ_CP074576:2704824-2705210 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T202432 NZ_CP099389:3666503-3666721 [Citrobacter braakii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT202432 WP_001280945.1 NZ_CP074576:2705200-2705529 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT202432 NZ_CP099389:3666101-3666475 [Citrobacter braakii]
ATGGATGAATACTCACCCAAAAGATATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGGTCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6