Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2457057..2457841 Replicon chromosome
Accession NZ_CP074576
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag KHZ70_RS11885 Protein ID WP_000613624.1
Coordinates 2457347..2457841 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID B1LI61
Locus tag KHZ70_RS11880 Protein ID WP_001110446.1
Coordinates 2457057..2457350 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS11870 (2452206) 2452206..2453165 - 960 WP_000846329.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
KHZ70_RS11875 (2453738) 2453738..2456923 + 3186 WP_000827395.1 ribonuclease E -
KHZ70_RS11880 (2457057) 2457057..2457350 + 294 WP_001110446.1 DUF1778 domain-containing protein Antitoxin
KHZ70_RS11885 (2457347) 2457347..2457841 + 495 WP_000613624.1 GNAT family N-acetyltransferase Toxin
KHZ70_RS11890 (2457936) 2457936..2458889 - 954 WP_001212762.1 flagellar hook-associated protein FlgL -
KHZ70_RS11895 (2458901) 2458901..2460544 - 1644 WP_000096482.1 flagellar hook-associated protein FlgK -
KHZ70_RS11900 (2460610) 2460610..2461551 - 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
KHZ70_RS11905 (2461551) 2461551..2462648 - 1098 WP_000589326.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(85-145)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18142.04 Da        Isoelectric Point: 7.7444

>T202431 WP_000613624.1 NZ_CP074576:2457347-2457841 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T202431 NZ_CP099389:c2854648-2854545 [Citrobacter braakii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11079.67 Da        Isoelectric Point: 10.5423

>AT202431 WP_001110446.1 NZ_CP074576:2457057-2457350 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT202431 NZ_CP099389:2854545-2854683 [Citrobacter braakii]
AAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829G775

References