Detailed information of TA system TA202430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2147942..2148578
• Replicon: chromosome
• Accession: NZ_CP074576
• Organism: Escherichia coli strain CS18F

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A5F1EUB3
• Locus tag: KHZ70_RS10330
• Protein ID: WP_000813796.1
• Coordinates: 2148402..2148578 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KHZ70_RS10325
• Protein ID: WP_076838470.1
• Coordinates: 2147942..2148358 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHZ70_RS10305 (2143094)	2143094..2144035	-	942	WP_001251307.1	ABC transporter permease	-
KHZ70_RS10310 (2144036)	2144036..2145049	-	1014	WP_213201980.1	ABC transporter ATP-binding protein	-
KHZ70_RS10315 (2145067)	2145067..2146212	-	1146	WP_000047448.1	ABC transporter substrate-binding protein	-
KHZ70_RS10320 (2146457)	2146457..2147863	-	1407	WP_000760605.1	PLP-dependent aminotransferase family protein	-
KHZ70_RS10325 (2147942)	2147942..2148358	-	417	WP_076838470.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KHZ70_RS10330 (2148402)	2148402..2148578	-	177	WP_000813796.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KHZ70_RS10335 (2148800)	2148800..2149030	+	231	WP_000494243.1	YncJ family protein	-
KHZ70_RS10340 (2149122)	2149122..2151083	-	1962	WP_001317809.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KHZ70_RS10345 (2151156)	2151156..2151692	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
KHZ70_RS10350 (2151745)	2151745..2152956	+	1212	WP_071593090.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6751.78 Da
• Isoelectric Point: 11.5666

>T202430 WP_000813796.1 NZ_CP074576:c2148578-2148402 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLN

Nucleotide

• Length: 177 bp

>T202430 NZ_CP099389:2406650-2406838 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGCTTACAGTCATCATCAATATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15219.59 Da
• Isoelectric Point: 4.5908

>AT202430 WP_076838470.1 NZ_CP074576:c2148358-2147942 [Escherichia coli]
MRYPVTLTPAPEGGYVVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT202430 NZ_CP099389:2406870-2407286 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCGCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
CCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCACTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACAATGTTGTAA

Structures

Toxin

• AlphaFold DB: A0A5F1EUB3