Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 972135..972760 Replicon chromosome
Accession NZ_CP074576
Organism Escherichia coli strain CS18F

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KHZ70_RS04565 Protein ID WP_000911329.1
Coordinates 972362..972760 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KHZ70_RS04560 Protein ID WP_000450524.1
Coordinates 972135..972362 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHZ70_RS04535 (967935) 967935..968405 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
KHZ70_RS04540 (968405) 968405..968977 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
KHZ70_RS04545 (969123) 969123..970001 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
KHZ70_RS04550 (970018) 970018..971052 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
KHZ70_RS04555 (971265) 971265..971978 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KHZ70_RS04560 (972135) 972135..972362 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KHZ70_RS04565 (972362) 972362..972760 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KHZ70_RS04570 (972907) 972907..973770 + 864 WP_001267498.1 neutral zinc metallopeptidase -
KHZ70_RS04575 (973785) 973785..975800 + 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
KHZ70_RS04580 (975874) 975874..976572 + 699 WP_000679819.1 esterase -
KHZ70_RS04585 (976653) 976653..976853 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T202420 WP_000911329.1 NZ_CP074576:972362-972760 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T202420 NZ_CP099387:4814877-4815188 [Citrobacter braakii]
ATGCATGTTATCAGCCGAAAACCTTTCAACGAGGCAATACTCCGTTTTCCAAACCACATGGCAGCACTCGTTGATTTGCT
GAATATTTTAGAGAAAAAAATGTTCCACACTCCTGAAGAGATGAAGCAGTACATACCTTCACTGGATAATTTTAAATACA
GAAATAAATGGTGGGTGATTAATGTTTCAGGTAACTGCTTGAGACTTATTGCCTATATTGATTTCAAACTGCAAAAGGTA
TTTGTGAAGCATATCGTTCACCATGCCGAGTATGACAGACTAACCACATACTATCGGGGTCACAAAGAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT202420 WP_000450524.1 NZ_CP074576:972135-972362 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT202420 NZ_CP099387:4815185-4815637 [Citrobacter braakii]
ATGAGAACGCATGAAAGCCATCAGATAGACACAGCCAGTGTAAAATTGATGATCGATACCTTTACCGATGCAGTGAAAAA
AATCCCCCTGTTAGGCAAAGAACAAAATGAGACAGAGTACCGAAAAGCACTCGCTTTGGTGGAGTTTCTGGTTGATCGTG
ACGACCTGGAAAACCCGTTGTTTGAGCTGCTTTCAGCTCGGATCCGCGACTACGAAAAACATGCGCCTGAGTTCAGTGCC
CTCAACCAACAACTGGAACATACTCCTCATGGCGTTACGTTACTGCGTACGCTCATGGATCAATATGGTCTCAAAGCAGC
AGATCTTGCCAACGAACTTGGTTCAAAATCCAATGTCAGCAACATCTTGAATGGTCGCAGAGCACTGACTGTAAGTCATA
TTAAAATGCTCGCAGAGCGCTTTAACCTTCCGGCAGACGCATTCATCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References