Detailed information of TA system TA202409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 510772..511408
• Replicon: chromosome
• Accession: NZ_CP074571
• Organism: Bacillus subtilis strain BYS2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KIH94_RS02595
• Protein ID: WP_003156187.1
• Coordinates: 511058..511408 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: KIH94_RS02590
• Protein ID: WP_003225183.1
• Coordinates: 510772..511053 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIH94_RS02570 (KIH94_02570)	507131..507730	-	600	WP_015482831.1	rhomboid family intramembrane serine protease	-
KIH94_RS02575 (KIH94_02575)	507825..508190	+	366	WP_015252768.1	holo-ACP synthase	-
KIH94_RS02580 (KIH94_02580)	508356..509372	+	1017	WP_080030712.1	outer membrane lipoprotein carrier protein LolA	-
KIH94_RS02585 (KIH94_02585)	509487..510656	+	1170	WP_015382833.1	alanine racemase	-
KIH94_RS02590 (KIH94_02590)	510772..511053	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KIH94_RS02595 (KIH94_02595)	511058..511408	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KIH94_RS02600 (KIH94_02600)	511524..512348	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
KIH94_RS02605 (KIH94_02605)	512353..512718	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
KIH94_RS02610 (KIH94_02610)	512722..513123	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
KIH94_RS02615 (KIH94_02615)	513135..514142	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
KIH94_RS02620 (KIH94_02620)	514204..514533	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
KIH94_RS02625 (KIH94_02625)	514530..515012	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
KIH94_RS02630 (KIH94_02630)	514978..515766	+	789	WP_015482833.1	RNA polymerase sigma factor SigB	-
KIH94_RS02635 (KIH94_02635)	515766..516365	+	600	WP_015382836.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T202409 WP_003156187.1 NZ_CP074571:511058-511408 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T202409 NZ_CP099386:3580487-3580705 [Citrobacter braakii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCGGAAT
TGACCATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT202409 WP_003225183.1 NZ_CP074571:510772-511053 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT202409 NZ_CP099386:3580085-3580459 [Citrobacter braakii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGGTCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32