Detailed information of TA system TA202404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1325965..1326557
• Replicon: chromosome
• Accession: NZ_CP074570
• Organism: Paraneptunicella aestuarii strain NBU2194

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: KIH87_RS05620
• Protein ID: WP_232360558.1
• Coordinates: 1326303..1326557 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KIH87_RS05615
• Protein ID: WP_232360557.1
• Coordinates: 1325965..1326294 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIH87_RS05575 (KIH87_05600)	1321663..1322409	+	747	WP_232360549.1	DUF481 domain-containing protein	-
KIH87_RS05580 (KIH87_05605)	1322656..1323111	+	456	WP_232360550.1	helix-turn-helix domain-containing protein	-
KIH87_RS05585 (KIH87_05610)	1323108..1323734	+	627	WP_232360551.1	helix-turn-helix domain-containing protein	-
KIH87_RS05590 (KIH87_05615)	1323741..1323935	-	195	WP_232360552.1	hypothetical protein	-
KIH87_RS05595 (KIH87_05620)	1323949..1324464	-	516	WP_232360553.1	hypothetical protein	-
KIH87_RS05600 (KIH87_05625)	1324505..1324807	-	303	WP_232360554.1	hypothetical protein	-
KIH87_RS05605 (KIH87_05630)	1325150..1325629	-	480	WP_232360555.1	putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase	-
KIH87_RS05610 (KIH87_05635)	1325687..1325893	-	207	WP_232360556.1	hypothetical protein	-
KIH87_RS05615 (KIH87_05640)	1325965..1326294	-	330	WP_232360557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
KIH87_RS05620 (KIH87_05645)	1326303..1326557	-	255	WP_232360558.1	type II toxin-antitoxin system HicA family toxin	Toxin
KIH87_RS05625 (KIH87_05650)	1326866..1327780	-	915	WP_232360559.1	hypothetical protein	-
KIH87_RS05630 (KIH87_05655)	1327862..1330219	-	2358	WP_232360560.1	EAL domain-containing protein	-
KIH87_RS05635 (KIH87_05660)	1330212..1330856	-	645	WP_232360561.1	methylamine utilization protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-74)

Antitoxin

• Uncharacterised protein family HicB (52-99)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9621.01 Da
• Isoelectric Point: 10.4689

>T202404 WP_232360558.1 NZ_CP074570:c1326557-1326303 [Paraneptunicella aestuarii]
MKRKYQKTLELIFNRPVSGNVKWKDIEQLFVELGAELTEGEGSRIGVRLFGERRVFHRPHPSPDTDKGAVSSIKRWLESN
GVTP

Nucleotide

• Length: 255 bp

>T202404 NZ_CP099385:4837116-4837427 [Citrobacter braakii]
ATGCATGTTATCAGCCGAAAACCTTTCAACGAGGCAATACTCCGTTTTCCAAACCACATGGCAGCACTCGTTGATTTGCT
GAATATTTTAGAGAAAAAAATGTTCCACACTCCTGAAGAGATGAAGCAGTACATACCTTCACTGGATAATTTTAAATACA
GAAATAAATGGTGGGTGATTAATGTTTCAGGTAACTGCTTGAGACTTATTGCCTATATTGATTTCAAACTGCAAAAGGTA
TTTGTGAAGCATATCGTTCACCATGCCGAGTATGACAGACTAACCACATACTATCGGGGTCACAAAGAATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11945.54 Da
• Isoelectric Point: 4.6005

>AT202404 WP_232360557.1 NZ_CP074570:c1326294-1325965 [Paraneptunicella aestuarii]
MKIDGYDAVIQYDPEIDMFRGEFINLNGGADFYASDISGLHKEGKLSLDMFLKMCLEDGVSPQKQYSGKFNVRISPELHE
SLVLKAAASGKSINQLVVDSISNTIANGQ

Nucleotide

• Length: 330 bp

>AT202404 NZ_CP099385:4837424-4837876 [Citrobacter braakii]
ATGAGAACGCATGAAAGTCATCATATAGACACAGCCAGTGTAAAATTGATGATCGATACCTTTACCGATGCAGTGAAAAA
AATCCCCCTGTTAGGCAAAGAACAAAATGAGACAGAGTACCGAAAAGCACTCGCTTTGGTGGAGTTTCTGGTTGATCGTG
ACGACCTGGAAAACCCGTTGTTTGAGCTGCTTTCAGCTCGGATCCGCGACTACGAAAAACATGCGCCTGAGTTCAGTGCC
CTCAACCAACAACTGGAACATACTCCTCATGGCGTTGCGTTACTGCGTACGCTCATGGATCAATATGGTCTCAAAGCAGC
AGATCTTGCCAACGAACTTGGTTCAAAATCTAATGTCAGCAACATCTTGAATGGTCGCAGAGCACTGACTGTAAGTCATA
TTAAAATGCTCGCAGAACGCTTTAACCTTCCGGCAGACGCATTCATCGAGTAA