Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 179983..180626 Replicon plasmid pCRE40_1
Accession NZ_CP074559
Organism Klebsiella pneumoniae strain S39_CRE40

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KHW03_RS28080 Protein ID WP_001044770.1
Coordinates 179983..180399 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KHW03_RS28085 Protein ID WP_001261282.1
Coordinates 180396..180626 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW03_RS28035 (KHW03_27945) 175277..175561 - 285 WP_001043731.1 hypothetical protein -
KHW03_RS28040 (KHW03_27950) 175597..175908 - 312 WP_000469598.1 hypothetical protein -
KHW03_RS28045 (KHW03_27955) 176114..176398 - 285 WP_001185481.1 hypothetical protein -
KHW03_RS28050 (KHW03_27960) 176498..177160 - 663 WP_000516402.1 division plane positioning ATPase MipZ -
KHW03_RS28055 (KHW03_27965) 177541..178203 + 663 WP_001549893.1 recombinase family protein -
KHW03_RS28060 (KHW03_27970) 178290..178529 + 240 WP_001549892.1 hypothetical protein -
KHW03_RS28065 178532..178888 + 357 WP_004199403.1 hypothetical protein -
KHW03_RS28070 (KHW03_27975) 178922..179626 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KHW03_RS28075 (KHW03_27980) 179691..179909 - 219 Protein_208 AAA family ATPase -
KHW03_RS28080 (KHW03_27985) 179983..180399 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KHW03_RS28085 (KHW03_27990) 180396..180626 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHW03_RS28090 (KHW03_27995) 180583..181043 + 461 Protein_211 hypothetical protein -
KHW03_RS28095 (KHW03_28000) 181171..181491 + 321 WP_032072060.1 hypothetical protein -
KHW03_RS28100 (KHW03_28005) 181540..182178 + 639 WP_022644887.1 hypothetical protein -
KHW03_RS28105 (KHW03_28010) 182181..182966 + 786 WP_022644886.1 site-specific integrase -
KHW03_RS28110 (KHW03_28015) 183024..183281 - 258 WP_032408936.1 hypothetical protein -
KHW03_RS28115 (KHW03_28020) 183410..183523 - 114 WP_032072094.1 hypothetical protein -
KHW03_RS28120 (KHW03_28025) 184154..184909 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 / dfrA14 / blaSHV-12 / blaKPC-3 / aac(6')-Ib - 1..308362 308362
- flank IS/Tn - - 178922..179626 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T202399 WP_001044770.1 NZ_CP074559:c180399-179983 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T202399 NZ_CP099385:3720826-3721044 [Citrobacter braakii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT202399 WP_001261282.1 NZ_CP074559:c180626-180396 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT202399 NZ_CP099385:3720424-3720798 [Citrobacter braakii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGGTCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References