Detailed information of TA system TA202398

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53667..54403
• Replicon: plasmid pCRE40_1
• Accession: NZ_CP074559
• Organism: Klebsiella pneumoniae strain S39_CRE40

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: KHW03_RS27315
• Protein ID: WP_003026803.1
• Coordinates: 53921..54403 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: KHW03_RS27310
• Protein ID: WP_003026799.1
• Coordinates: 53667..53933 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KHW03_RS27265 (KHW03_27185)	49729..50091	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
KHW03_RS27270 (KHW03_27190)	50141..50491	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
KHW03_RS27275 (KHW03_27195)	50849..51118	+	270	WP_004152102.1	hypothetical protein	-
KHW03_RS27280 (KHW03_27200)	51106..51681	+	576	WP_004152103.1	hypothetical protein	-
KHW03_RS27285 (KHW03_27205)	51712..52206	+	495	WP_004152104.1	DNA-binding protein	-
KHW03_RS27290 (KHW03_27210)	52250..52618	+	369	WP_004152105.1	hypothetical protein	-
KHW03_RS27295 (KHW03_27215)	52652..52855	+	204	WP_004152106.1	HHA domain-containing protein	-
KHW03_RS27300 (KHW03_27220)	52904..53161	+	258	WP_004152107.1	hypothetical protein	-
KHW03_RS27305 (KHW03_27225)	53237..53491	+	255	WP_004152108.1	hypothetical protein	-
KHW03_RS27310 (KHW03_27230)	53667..53933	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
KHW03_RS27315 (KHW03_27235)	53921..54403	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
KHW03_RS27320 (KHW03_27240)	54615..55961	+	1347	WP_020314316.1	ISNCY family transposase	-
KHW03_RS27325	56122..56253	+	132	WP_004218042.1	hypothetical protein	-
KHW03_RS27330 (KHW03_27245)	56462..57637	-	1176	Protein_59	IS3-like element ISKpn38 family transposase	-
KHW03_RS27335 (KHW03_27250)	57814..58776	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
KHW03_RS27340 (KHW03_27255)	58763..59251	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 / dfrA14 / blaSHV-12 / blaKPC-3 / aac(6')-Ib	-	1..308362	308362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T202398 WP_003026803.1 NZ_CP074559:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T202398 NZ_CP099385:c2915728-2915625 [Citrobacter braakii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT202398 WP_003026799.1 NZ_CP074559:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT202398 NZ_CP099385:2915622-2915763 [Citrobacter braakii]
GATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGG
CATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66