Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5392695..5393320 Replicon chromosome
Accession NZ_CP074558
Organism Klebsiella pneumoniae strain S39_CRE40

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHW03_RS26670 Protein ID WP_002882817.1
Coordinates 5392695..5393078 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHW03_RS26675 Protein ID WP_004150355.1
Coordinates 5393078..5393320 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW03_RS26655 (KHW03_26580) 5390061..5390963 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHW03_RS26660 (KHW03_26585) 5390960..5391595 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHW03_RS26665 (KHW03_26590) 5391592..5392521 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHW03_RS26670 (KHW03_26595) 5392695..5393078 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHW03_RS26675 (KHW03_26600) 5393078..5393320 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHW03_RS26680 (KHW03_26605) 5393525..5394442 + 918 WP_002882812.1 alpha/beta hydrolase -
KHW03_RS26685 (KHW03_26610) 5394456..5395397 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHW03_RS26690 (KHW03_26615) 5395442..5395879 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHW03_RS26695 (KHW03_26620) 5395876..5396736 - 861 WP_002882807.1 virulence factor BrkB family protein -
KHW03_RS26700 (KHW03_26625) 5396730..5397329 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202397 WP_002882817.1 NZ_CP074558:c5393078-5392695 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202397 NZ_CP099385:2414061-2414249 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGCTTACAGTCATCATCAATATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202397 WP_004150355.1 NZ_CP074558:c5393320-5393078 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202397 NZ_CP099385:2414281-2414697 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCGCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
TCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCGCTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATTGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACAATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References