Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4805335..4806145 Replicon chromosome
Accession NZ_CP074558
Organism Klebsiella pneumoniae strain S39_CRE40

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KHW03_RS23845 Protein ID WP_002887280.1
Coordinates 4805335..4805868 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHW03_RS23850 Protein ID WP_002887278.1
Coordinates 4805879..4806145 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW03_RS23840 (KHW03_23775) 4804166..4805287 + 1122 WP_002887282.1 cupin domain-containing protein -
KHW03_RS23845 (KHW03_23780) 4805335..4805868 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KHW03_RS23850 (KHW03_23785) 4805879..4806145 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHW03_RS23855 (KHW03_23790) 4806248..4807681 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KHW03_RS23860 (KHW03_23795) 4807671..4808354 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KHW03_RS23865 (KHW03_23800) 4808526..4809911 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KHW03_RS23870 (KHW03_23805) 4809929..4810273 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T202394 WP_002887280.1 NZ_CP074558:c4805868-4805335 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202394 NZ_CP099384:4679772-4680083 [Citrobacter braakii]
ATGCATGTTATCAGCCGAAAACCTTTCAACGAGGCAATACTCCGTTTTCCAAACCACATGGCAGCACTCGTTGATTTGCT
GAATATTTTAGAGAAAAAAATGTTCCACACTCCTGAAGAGATGAAGCAGTACATACCTTCACTGGATAATTTTAAATACA
GAAATAAATGGTGGGTGATTAATGTTTCAGGTAACTGCTTGAGACTTATTGCCTATATTGATTTCAAACTGCAAAAGGTA
TTTGTGAAGCATATCGTTCACCATGCCGAGTATGACAGACTAACCACATACTATCGGGGTCACAAAGAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202394 WP_002887278.1 NZ_CP074558:c4806145-4805879 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202394 NZ_CP099384:4680080-4680532 [Citrobacter braakii]
ATGAGAACGCATGAAAGCCATCAGATAGACACAGCCAGTGTAAAATTGATGATCGATACCTTTACCGATGCAGTGAAAAA
AATCCCCCTGTTAGGCAAAGAACAAAATGAGACAGAGTACCGAAAAGCACTCGCTTTGGTGGAGTTTCTGGTTGATCGTG
ACGACCTGGAAAACCCGTTGTTTGAGCTGCTTTCAGCTCGGATACGCGACTACGAAAAACATGCGCCTGAGTTCAGTGCC
CTCAACCAACAACTGGAACATACTCCTCATGGCGTTGCGTTACTGCGTACGCTCATGGATCAATATGGTCTCAAAGCAGC
AGATCTTGCCAACGAACTTGGTTCAAAATCCAATGTCAGCAACATCTTGAATGGTCGCAGAGCACTGACTGTAAGTCATA
TTAAAATGCTCGCAGAACGCTTTAACCTTCCGGCAGACGCATTCATCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References