Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4192654..4193273 Replicon chromosome
Accession NZ_CP074558
Organism Klebsiella pneumoniae strain S39_CRE40

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KHW03_RS20900 Protein ID WP_002892050.1
Coordinates 4193055..4193273 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KHW03_RS20895 Protein ID WP_002892066.1
Coordinates 4192654..4193028 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW03_RS20885 (KHW03_20820) 4187806..4188999 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHW03_RS20890 (KHW03_20825) 4189022..4192168 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KHW03_RS20895 (KHW03_20830) 4192654..4193028 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KHW03_RS20900 (KHW03_20835) 4193055..4193273 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KHW03_RS20905 (KHW03_20840) 4193432..4193998 + 567 WP_002892030.1 maltose O-acetyltransferase -
KHW03_RS20910 (KHW03_20845) 4193970..4194110 - 141 WP_004147370.1 hypothetical protein -
KHW03_RS20915 (KHW03_20850) 4194131..4194601 + 471 WP_002892026.1 YlaC family protein -
KHW03_RS20920 (KHW03_20855) 4194576..4196027 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KHW03_RS20925 (KHW03_20860) 4196128..4196826 + 699 WP_002892021.1 GNAT family protein -
KHW03_RS20930 (KHW03_20865) 4196823..4196963 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KHW03_RS20935 (KHW03_20870) 4196963..4197226 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202393 WP_002892050.1 NZ_CP074558:4193055-4193273 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202393 NZ_CP099384:c4678310-4677936 [Citrobacter braakii]
ATGGTCACAGGATCTGCGCTTTTTGATACCAACATCCTGATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGAGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATCGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT202393 WP_002892066.1 NZ_CP074558:4192654-4193028 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202393 NZ_CP099384:c4678552-4678310 [Citrobacter braakii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCTGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGCGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References