Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1895578..1897166 Replicon chromosome
Accession NZ_CP074558
Organism Klebsiella pneumoniae strain S39_CRE40

Toxin (Protein)


Gene name hipA Uniprot ID W8UI36
Locus tag KHW03_RS09450 Protein ID WP_002904792.1
Coordinates 1895844..1897166 (+) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag KHW03_RS09445 Protein ID WP_002904790.1
Coordinates 1895578..1895844 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW03_RS09420 (KHW03_09415) 1890728..1891651 + 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -
KHW03_RS09425 (KHW03_09420) 1891632..1892786 - 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
KHW03_RS09430 (KHW03_09425) 1893132..1894511 + 1380 WP_002904785.1 nucleobase:cation symporter-2 family protein -
KHW03_RS09435 (KHW03_09430) 1894489..1894989 + 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
KHW03_RS09440 (KHW03_09435) 1894986..1895312 + 327 WP_002904788.1 hydroxyisourate hydrolase -
KHW03_RS09445 (KHW03_09440) 1895578..1895844 + 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
KHW03_RS09450 (KHW03_09445) 1895844..1897166 + 1323 WP_002904792.1 type II toxin-antitoxin system HipA family toxin Toxin
KHW03_RS09455 (KHW03_09450) 1897183..1898475 - 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
KHW03_RS09460 (KHW03_09455) 1898617..1899621 - 1005 WP_002904798.1 fimbrial protein -
KHW03_RS09465 (KHW03_09460) 1899609..1902110 - 2502 WP_004151634.1 fimbria/pilus outer membrane usher protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T202384 WP_002904792.1 NZ_CP074558:1895844-1897166 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T202384 NZ_CP099382:c4964115-4963741 [Citrobacter braakii]
ATGGCCACAGGATCTGCGCTTTTTGATACCAACATCCTCATCGACCTGTTTAGCGGGCGAAGTGAAGCAAAACTAGCGAT
AGAAACATGGCCGCCGCAGAACGCAATCAGCCTCATTACATGGATGGAGTTGATGGTGGGCGCAAAAAAATATCATCAGG
AATCTCGCACACGCGTTGCGCTAGGCGCTTTTAATGTGATAGGTGTTTCACAGGATATTGCTGAACGAAGCGTGAGTATC
AGGCAGGAATATGGGATGAAATTGCCGGATGCCATCATTTTGGCCACTGCGCAGATTCATCGTCTTACACTGGTTACGCG
TAATACCAGGGATTTTGCAGGCATATCCGGCGTGGTCACACCGTATACGTTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT202384 WP_002904790.1 NZ_CP074558:1895578-1895844 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT202384 NZ_CP099382:c4964357-4964115 [Citrobacter braakii]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGAAGTGATCAAACGTCTTGATGATCTCAAGGT
GCAGCGTAATCTGCCGCGGGCCGAGCTTTTGCGGGAGGCTGTTGAACAGTATCTTGAGAGGCAGGATCAAGCTGAAACGA
CAATTTCACGTGCTCTTGGGCTATGGCAAGGTTGTGAGGAAGATGGCGTTGATTATCAGCGTAAACTCCGTGAGGAATGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CEC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References