Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5811..6336 Replicon plasmid pCRE38_2
Accession NZ_CP074555
Organism Klebsiella pneumoniae strain S37_CRE38

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag KHW02_RS27715 Protein ID WP_001568026.1
Coordinates 5811..6116 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag KHW02_RS27720 Protein ID WP_001568025.1
Coordinates 6118..6336 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW02_RS27685 (KHW02_27590) 1519..2145 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
KHW02_RS27690 2142..2444 + 303 WP_004197636.1 hypothetical protein -
KHW02_RS27695 (KHW02_27600) 2892..3686 - 795 WP_004197635.1 site-specific integrase -
KHW02_RS27700 (KHW02_27605) 3884..4900 - 1017 WP_020315256.1 hypothetical protein -
KHW02_RS27705 (KHW02_27610) 4897..5220 - 324 WP_022644730.1 hypothetical protein -
KHW02_RS27710 (KHW02_27615) 5247..5642 - 396 WP_017899885.1 hypothetical protein -
KHW02_RS27715 (KHW02_27620) 5811..6116 - 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
KHW02_RS27720 (KHW02_27625) 6118..6336 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KHW02_RS27725 (KHW02_27630) 6889..7578 + 690 WP_004193995.1 hypothetical protein -
KHW02_RS27730 (KHW02_27635) 7610..8299 - 690 WP_020315560.1 RES family NAD+ phosphorylase -
KHW02_RS27735 (KHW02_27640) 8736..9005 + 270 WP_020804424.1 hypothetical protein -
KHW02_RS27740 (KHW02_27645) 8993..9568 + 576 WP_020317495.1 hypothetical protein -
KHW02_RS27745 (KHW02_27650) 9599..10093 + 495 WP_020802777.1 hypothetical protein -
KHW02_RS27750 (KHW02_27655) 10137..10505 + 369 WP_004197807.1 hypothetical protein -
KHW02_RS27755 (KHW02_27660) 10539..10742 + 204 WP_004197808.1 HHA domain-containing protein -
KHW02_RS27760 (KHW02_27665) 10756..10959 + 204 WP_004197809.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..36101 36101


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T202379 WP_001568026.1 NZ_CP074555:c6116-5811 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T202379 NZ_CP099382:c2943269-2943166 [Citrobacter braakii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT202379 WP_001568025.1 NZ_CP074555:c6336-6118 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT202379 NZ_CP099382:2943164-2943304 [Citrobacter braakii]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References