Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 72073..72716 Replicon plasmid pCRE38_1
Accession NZ_CP074554
Organism Klebsiella pneumoniae strain S37_CRE38

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag KHW02_RS27225 Protein ID WP_001044770.1
Coordinates 72073..72489 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag KHW02_RS27230 Protein ID WP_001261282.1
Coordinates 72486..72716 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW02_RS27180 67363..67428 - 66 Protein_76 EAL domain-containing protein -
KHW02_RS27185 67538..67654 - 117 Protein_77 plasmid mobilization relaxosome protein MobC -
KHW02_RS27190 (KHW02_27120) 67875..68348 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
KHW02_RS27195 (KHW02_27125) 68504..69271 + 768 Protein_79 class 1 integron integrase IntI1 -
KHW02_RS27200 (KHW02_27130) 69324..70028 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KHW02_RS27205 70093..70176 - 84 Protein_81 hypothetical protein -
KHW02_RS27210 (KHW02_27135) 70292..70480 + 189 WP_000957857.1 hypothetical protein -
KHW02_RS27215 (KHW02_27140) 70490..71689 + 1200 WP_000948429.1 IS91 family transposase -
KHW02_RS27220 (KHW02_27145) 71856..71999 - 144 Protein_84 AAA family ATPase -
KHW02_RS27225 (KHW02_27150) 72073..72489 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
KHW02_RS27230 (KHW02_27155) 72486..72716 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KHW02_RS27235 (KHW02_27160) 72673..73133 + 461 Protein_87 hypothetical protein -
KHW02_RS27240 (KHW02_27165) 73261..73581 + 321 WP_032072060.1 hypothetical protein -
KHW02_RS27245 (KHW02_27170) 73790..73978 + 189 WP_000957857.1 hypothetical protein -
KHW02_RS27250 (KHW02_27175) 73988..75187 + 1200 WP_000948429.1 IS91 family transposase -
KHW02_RS27255 (KHW02_27180) 75360..75482 + 123 Protein_91 phage integrase family protein -
KHW02_RS27260 (KHW02_27185) 75540..75797 - 258 WP_032408936.1 hypothetical protein -
KHW02_RS27265 (KHW02_27190) 75926..76039 - 114 WP_032072094.1 hypothetical protein -
KHW02_RS27270 (KHW02_27195) 76670..77425 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..142024 142024
- inside IScluster/Tn blaKPC-3 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 38739..79848 41109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T202378 WP_001044770.1 NZ_CP074554:c72489-72073 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T202378 NZ_CP099382:2459457-2459645 [Citrobacter braakii]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTGGCTGTTACAAACGGCTCAAATCATCTGAAGTTGAG
GTACCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCTGGTGATGAAATAAAAGAAGCCTTGCGAAAATCGATCCTGAAGC
AGCTTGGTTTACAGTCATCATCAATATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT202378 WP_001261282.1 NZ_CP074554:c72716-72486 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT202378 NZ_CP099382:2459677-2460093 [Citrobacter braakii]
ATGCGTTATCCCGTTAATCTAATCCCCTCCGTGGAAGGTGGATTCGTGGTTTCTTTCCCGGATATTCCTGAAGCATTAAC
CCAGGGCGAAACCCGTCATGGGGCTTTGCAGGCTGCGCAGTCTGCATTGGTGACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCGGCGGTTGGCACTGAAGGTGACTATGTGGAAATCCCCCTGAGCATTGCGTCGAAGGTT
CTTTTGTTAAATGCCTTTCTTGAATCAAAAATCACCCAGCAGGAGCTGGCAAACAGGATCGGCAGGCCTAAGCAGGAAAT
TACCCGTTTGTTCGATTTAAAGCACGCGACAAAAATTGATGCTGTGCAAATAGCTGCACGCGCCTTAGGCAAGGAGCTGT
CAGTAACAATGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References