Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5365058..5365683 Replicon chromosome
Accession NZ_CP074553
Organism Klebsiella pneumoniae strain S37_CRE38

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHW02_RS26435 Protein ID WP_002882817.1
Coordinates 5365058..5365441 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHW02_RS26440 Protein ID WP_004150355.1
Coordinates 5365441..5365683 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW02_RS26420 (KHW02_26350) 5362424..5363326 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHW02_RS26425 (KHW02_26355) 5363323..5363958 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHW02_RS26430 (KHW02_26360) 5363955..5364884 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHW02_RS26435 (KHW02_26365) 5365058..5365441 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHW02_RS26440 (KHW02_26370) 5365441..5365683 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHW02_RS26445 (KHW02_26375) 5365888..5366805 + 918 WP_002882812.1 alpha/beta hydrolase -
KHW02_RS26450 (KHW02_26380) 5366819..5367760 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHW02_RS26455 (KHW02_26385) 5367805..5368242 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHW02_RS26460 (KHW02_26390) 5368239..5369099 - 861 WP_002882807.1 virulence factor BrkB family protein -
KHW02_RS26465 (KHW02_26395) 5369093..5369692 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202377 WP_002882817.1 NZ_CP074553:c5365441-5365058 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202377 NZ_CP099382:876931-877338 [Citrobacter braakii]
GTGGTCCAGTGGCAATCTGATTTACGCGTTTCATGGCGCGCGCAATGGCTTTCGTTGCTCATTCATGGGCTGGTCGCGGT
ATTTATTTTATTGATGCCCTGGCCATTGAGCTACACCCCATTATGGCTGGTTCTGCTCTCTCTGGTGGTGTTCGATAGCG
TCCGTAGCCAGCGCCGAATCAATGCCTGTCAGGGCGAGATTCGGCTGTTGATGGACGGTCGTCTGCGCTGGCAAGGGCAA
GAGTGGACTATTGCTAGCGCACCCTGGATGGTCAAAACAGGCATGATGTTGCGTTTACGTTCCGATGCCGGAAAACGTCA
GCATTTGTGGCTGGCGGCAGACAGTATGGATGACGCGGAGTGGCGGGATTTGCGGCGGTTAATACTGCAACAGGCGAAGC
AGGGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202377 WP_004150355.1 NZ_CP074553:c5365683-5365441 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202377 NZ_CP099382:876684-876950 [Citrobacter braakii]
ATGGATATTAACAACAAAGCACGTATTCATTGGGCATGCCGTCGCGGTATGCGTGAGCTGGACATCTCGATCATGCCGTT
TTTCGAACATGAATATGACAGCTTAAGTGATGAAGAAAAACGTATCTTTATCCGTCTGCTAGAATGTGATGATCCAGACT
TATTTAACTGGTTGATGAATCATGGGAAACCGGCTGATGCTCAGCTAGAGCAGATGGTGCGACTTATCCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References