Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4778058..4778868 Replicon chromosome
Accession NZ_CP074553
Organism Klebsiella pneumoniae strain S37_CRE38

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KHW02_RS23615 Protein ID WP_002887280.1
Coordinates 4778058..4778591 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KHW02_RS23620 Protein ID WP_002887278.1
Coordinates 4778602..4778868 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW02_RS23610 (KHW02_23550) 4776889..4778010 + 1122 WP_002887282.1 cupin domain-containing protein -
KHW02_RS23615 (KHW02_23555) 4778058..4778591 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KHW02_RS23620 (KHW02_23560) 4778602..4778868 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KHW02_RS23625 (KHW02_23565) 4778971..4780404 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
KHW02_RS23630 (KHW02_23570) 4780394..4781077 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
KHW02_RS23635 (KHW02_23575) 4781249..4782634 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
KHW02_RS23640 (KHW02_23580) 4782652..4782996 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T202374 WP_002887280.1 NZ_CP074553:c4778591-4778058 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T202374 NZ_CP099381:c4362664-4362344 [Citrobacter braakii]
ATGCACATTTCATCTGTACCGCCCACGGTGCCGGTTTCGTCACGCCTGTCACCCGTACAGGTGTGGAAGCAACTGTTAAC
GTATCTGCTGGAACACCATTACGGCCTGACACTCAACGACACGCCATTTCATGACGGCGCGGCCATTGAGGATCATATCG
ATGCGGGAATAACGCTTGCCGATGCGGTTAATTTCCTAGTGGAACGTTATGAACTGGTACGCATCGACCGCAAAGGATTT
ACATGGCAGGAGCAGACGCCATTCTTGACCACCACTGATATTCTCAGAGCCAGGCGAGCTACCGGATTAATAAATACCTG
A

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT202374 WP_002887278.1 NZ_CP074553:c4778868-4778602 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT202374 NZ_CP099381:c4363024-4362701 [Citrobacter braakii]
ATGTCAATCACACCACCTCATGAATGGGGGCTACAAAGCGATATCACCCCACGATTTGGCGCTAGATTAGTTCAGGAAGG
CAACCGGTTGTATTACCCGGCTGACAGGGCTGGATTGATGGGCAGTTTTAGCCCGATACACCTGCAGAAATTAGATCTGG
CTTTTCCGCTATTTGTAGAAAAGCTGGAAAATATGCTGCGCGCTGGCGAACTAAATCCACATCAGCAACATCAGGTGACC
ATTCAACTTGCTAGTTTAACCTGTATAGCTGATACCCGTGGATGCTGCGGTTACGTTTACATTTCTATTTATCCAACTCC
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References