Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4165369..4165988 Replicon chromosome
Accession NZ_CP074553
Organism Klebsiella pneumoniae strain S37_CRE38

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KHW02_RS20670 Protein ID WP_002892050.1
Coordinates 4165770..4165988 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KHW02_RS20665 Protein ID WP_002892066.1
Coordinates 4165369..4165743 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW02_RS20655 (KHW02_20595) 4160521..4161714 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHW02_RS20660 (KHW02_20600) 4161737..4164883 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KHW02_RS20665 (KHW02_20605) 4165369..4165743 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KHW02_RS20670 (KHW02_20610) 4165770..4165988 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KHW02_RS20675 (KHW02_20615) 4166147..4166713 + 567 WP_002892030.1 maltose O-acetyltransferase -
KHW02_RS20680 (KHW02_20620) 4166685..4166825 - 141 WP_004147370.1 hypothetical protein -
KHW02_RS20685 (KHW02_20625) 4166846..4167316 + 471 WP_002892026.1 YlaC family protein -
KHW02_RS20690 (KHW02_20630) 4167291..4168742 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
KHW02_RS20695 (KHW02_20635) 4168843..4169541 + 699 WP_002892021.1 GNAT family protein -
KHW02_RS20700 (KHW02_20640) 4169538..4169678 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KHW02_RS20705 (KHW02_20645) 4169678..4169941 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T202373 WP_002892050.1 NZ_CP074553:4165770-4165988 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202373 NZ_CP099381:3724163-3724381 [Citrobacter braakii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACTATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT202373 WP_002892066.1 NZ_CP074553:4165369-4165743 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202373 NZ_CP099381:3723761-3724135 [Citrobacter braakii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGGTCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References