Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4132573..4133192 Replicon chromosome
Accession NZ_CP074545
Organism Klebsiella aerogenes strain S33_CRE34

Toxin (Protein)


Gene name Hha Uniprot ID A0A0H3FXE2
Locus tag KHW00_RS20170 Protein ID WP_015367918.1
Coordinates 4132974..4133192 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H3FPM3
Locus tag KHW00_RS20165 Protein ID WP_015367917.1
Coordinates 4132573..4132947 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW00_RS20155 (KHW00_20080) 4127732..4128931 + 1200 WP_020079451.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KHW00_RS20160 (KHW00_20085) 4128954..4132100 + 3147 WP_015367916.1 multidrug efflux RND transporter permease subunit AcrB -
KHW00_RS20165 (KHW00_20090) 4132573..4132947 + 375 WP_015367917.1 Hha toxicity modulator TomB Antitoxin
KHW00_RS20170 (KHW00_20095) 4132974..4133192 + 219 WP_015367918.1 HHA domain-containing protein Toxin
KHW00_RS20175 (KHW00_20100) 4133324..4133887 + 564 WP_015367919.1 maltose O-acetyltransferase -
KHW00_RS20180 (KHW00_20105) 4134013..4134477 + 465 WP_015367920.1 YlaC family protein -
KHW00_RS20185 (KHW00_20110) 4134452..4135906 - 1455 WP_015367921.1 PLP-dependent aminotransferase family protein -
KHW00_RS20190 (KHW00_20115) 4136009..4136719 + 711 WP_015367922.1 GNAT family protein -
KHW00_RS20195 (KHW00_20120) 4136716..4136856 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
KHW00_RS20200 (KHW00_20125) 4136859..4137119 - 261 WP_015367923.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8569.96 Da        Isoelectric Point: 9.4828

>T202358 WP_015367918.1 NZ_CP074545:4132974-4133192 [Klebsiella aerogenes]
MSGKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T202358 NZ_CP099378:c4153618-4153235 [Citrobacter braakii]
ATGCAGACAAAACTCATGACCCCGTTGGGGGCGGCACATACCTGCCTGACCCCTATAGCCATATGGCAGACTTTGCTGAC
CCAATTACTGGGGCAGCACTATGGTCTTCAACTACACGATACTCCATTTAGCGATGATGACGTGATTCAAAGCCATATCG
ACGCCGGAATTACCTTAGTTGATGCCCTAAATTTTATTGTCGAAAAATACGAACTGGTTCGGACAGATCGATCGGAGTTT
ACAATCCTGGAACGATCACCCTTTATTACCGCCGTAGATATTCTAAGAGCCAGAAAGGTAACCGATCTGAGTCTGCGAAG
CGGTTACAGAATGATAAGCCGTATTACTCAGGGTAAAGGCTCTGACCAGGAGAATGAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14464.21 Da        Isoelectric Point: 4.9045

>AT202358 WP_015367917.1 NZ_CP074545:4132573-4132947 [Klebsiella aerogenes]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNRGWVNDPTSAVNLQLNELIEHIATYALNYKIKYAEDNKLISQLDEYL
DDTFMLFSSYGINTSDLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT202358 NZ_CP099378:c4154046-4153690 [Citrobacter braakii]
ATGTCGAAAAAGACACTAACAATGGACGATGACGCAACTGAACCATGGTGGGGGCTTAAGTGCGATACCACTTCATGCTT
TGGGTCCCGTCTGGTGCAAGAAGGTAACCGGCTACATTATCTGGCTGACCGTGCCTGCCTAATGGGTCAGTTCAGCGAAG
CGGATTTGCACCATCTTGACCAGGCATTTCCGGTGCTGCTGAAGCAAATAGAACTCATGCTCACCAGCGGCGAACTGAAT
CCCCGATACCAGCATTGCGTCACTCTCTATGCAAAGGGGCTGACCTGCGAAGCCGATACGCTGGGTTCTTGTGGCTATGT
TTATCTGGCTGTGTACCCAACGCTTCACACCAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FXE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FPM3

References