Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 77438..78459 Replicon chromosome
Accession NZ_CP074545
Organism Klebsiella aerogenes strain S33_CRE34

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A0G9UXE3
Locus tag KHW00_RS00390 Protein ID WP_032706547.1
Coordinates 77884..78459 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A0G9R618
Locus tag KHW00_RS00385 Protein ID WP_015369110.1
Coordinates 77438..77884 (+) Length 149 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHW00_RS00360 (KHW00_00360) 72959..73738 + 780 WP_015369105.1 MBL fold metallo-hydrolase -
KHW00_RS00365 (KHW00_00365) 73760..74713 + 954 WP_032706577.1 helix-turn-helix domain-containing protein -
KHW00_RS00370 (KHW00_00370) 74710..75759 - 1050 WP_015369107.1 NAD(P)-dependent alcohol dehydrogenase -
KHW00_RS00375 (KHW00_00375) 75917..76219 - 303 WP_015369108.1 putative quinol monooxygenase -
KHW00_RS00380 (KHW00_00380) 76220..77227 - 1008 WP_015369109.1 zinc-binding alcohol dehydrogenase family protein -
KHW00_RS00385 (KHW00_00385) 77438..77884 + 447 WP_015369110.1 helix-turn-helix domain-containing protein Antitoxin
KHW00_RS00390 (KHW00_00390) 77884..78459 + 576 WP_032706547.1 PIN domain-containing protein Toxin
KHW00_RS00395 (KHW00_00395) 78764..79960 + 1197 WP_015369112.1 hypothetical protein -
KHW00_RS00400 (KHW00_00400) 80137..81174 - 1038 WP_032706544.1 RhuM family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-115)

Antitoxin

SinI-like, DNA-binding domain

(79-126)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21688.29 Da        Isoelectric Point: 6.2900

>T202338 WP_032706547.1 NZ_CP074545:77884-78459 [Klebsiella aerogenes]
MRHSPYPVILDACVLYPARLRDLLMHLGIAGLYQPKWSRFIHDEWCRNLLINRPDIAPEALRRTVDLMNAALPDANVTGF
AGLINGLVLPDPDDRHVLAAAIRAKAEIIVTLNHKDFPAESLLPFEIETLHPDVFISDLFDLNHALALEAVRRQRQSLKH
PPMTVDEFLEMLLKQGLPMTVKELANYQYAI

Download         Length: 576 bp

>T202338 NZ_CP099376:2079727-2079830 [Citrobacter braakii]
GGCAGGGTAACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 149 a.a.        Molecular weight: 16699.26 Da        Isoelectric Point: 6.2257

>AT202338 WP_015369110.1 NZ_CP074545:77438-77884 [Klebsiella aerogenes]
MTKLNLPDPLESELAQRGQRELAAYLSTHLETQRIAIVGEDDKTHTIELPTSALTMLMEILGELACGNAVQIVPVHAELT
TQEAANILNVSRPHMVKLLEARKLPFHKTGRHRRVLFADLMEYKKRREQESLDAMQALADQAQDLGMY

Download         Length: 447 bp

>AT202338 NZ_CP099376:c2079830-2079692 [Citrobacter braakii]
AAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TAATGCAGGCTAAGTTACCCTGCCATTTAAGAATAGATGACAGCGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9UXE3

Antitoxin

Source ID Structure
AlphaFold DB A0A0G9R618

References