Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53667..54403 Replicon plasmid pCRE24_1
Accession NZ_CP074542
Organism Klebsiella pneumoniae strain S24_CRE24

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KHV99_RS27530 Protein ID WP_003026803.1
Coordinates 53921..54403 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KHV99_RS27525 Protein ID WP_003026799.1
Coordinates 53667..53933 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV99_RS27480 (KHV99_27405) 49729..50091 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KHV99_RS27485 (KHV99_27410) 50141..50491 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KHV99_RS27490 (KHV99_27415) 50849..51118 + 270 WP_004152102.1 hypothetical protein -
KHV99_RS27495 (KHV99_27420) 51106..51681 + 576 WP_004152103.1 hypothetical protein -
KHV99_RS27500 (KHV99_27425) 51712..52206 + 495 WP_004152104.1 DNA-binding protein -
KHV99_RS27505 (KHV99_27430) 52250..52618 + 369 WP_004152105.1 hypothetical protein -
KHV99_RS27510 (KHV99_27435) 52652..52855 + 204 WP_004152106.1 HHA domain-containing protein -
KHV99_RS27515 (KHV99_27440) 52904..53161 + 258 WP_004152107.1 hypothetical protein -
KHV99_RS27520 (KHV99_27445) 53237..53491 + 255 WP_004152108.1 hypothetical protein -
KHV99_RS27525 (KHV99_27450) 53667..53933 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KHV99_RS27530 (KHV99_27455) 53921..54403 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KHV99_RS27535 (KHV99_27460) 54615..55961 + 1347 WP_020314316.1 ISNCY family transposase -
KHV99_RS27540 56122..56253 + 132 WP_004218042.1 hypothetical protein -
KHV99_RS27545 (KHV99_27465) 56462..57637 - 1176 Protein_59 IS3-like element ISKpn38 family transposase -
KHV99_RS27550 (KHV99_27470) 57814..58776 - 963 WP_004152113.1 zinc metalloprotease HtpX -
KHV99_RS27555 (KHV99_27475) 58763..59251 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 / aac(6')-Ib - 1..217630 217630


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T202336 WP_003026803.1 NZ_CP074542:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T202336 NZ_CP099376:1938034-1938366 [Citrobacter braakii]
ATGGACAGAGGAGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTGCTTAT
CGTTTCTCCTGCATCATTTAATAAGTTCACCCGGCTACCCGTTGTCGTCCCCGTCACCAGCGGCGGAAACTTTGCCCGCG
CAGCCGGTTTTACCGTCTCCCTGGACGGTGTGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAACCCAGAACGATT
GATATGGGGGCCCGGAACGGTAAGTGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGGCTGGAAGC
CATTCTAAGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT202336 WP_003026799.1 NZ_CP074542:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT202336 NZ_CP099376:1937776-1938033 [Citrobacter braakii]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGCGCT
GTCGACAGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGACCTCGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCGCACGCTGAAATGAGTGATGAGGATAGGGAATGGATTGATGCGCCTGCAGTG
GGCAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References