Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5429635..5430260 Replicon chromosome
Accession NZ_CP074541
Organism Klebsiella pneumoniae strain S24_CRE24

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KHV99_RS26885 Protein ID WP_002882817.1
Coordinates 5429635..5430018 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KHV99_RS26890 Protein ID WP_004150355.1
Coordinates 5430018..5430260 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KHV99_RS26870 (KHV99_26800) 5427001..5427903 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KHV99_RS26875 (KHV99_26805) 5427900..5428535 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KHV99_RS26880 (KHV99_26810) 5428532..5429461 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KHV99_RS26885 (KHV99_26815) 5429635..5430018 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KHV99_RS26890 (KHV99_26820) 5430018..5430260 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KHV99_RS26895 (KHV99_26825) 5430465..5431382 + 918 WP_002882812.1 alpha/beta hydrolase -
KHV99_RS26900 (KHV99_26830) 5431396..5432337 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KHV99_RS26905 (KHV99_26835) 5432382..5432819 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KHV99_RS26910 (KHV99_26840) 5432816..5433676 - 861 WP_002882807.1 virulence factor BrkB family protein -
KHV99_RS26915 (KHV99_26845) 5433670..5434269 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T202335 WP_002882817.1 NZ_CP074541:c5430018-5429635 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T202335 NZ_CP099376:c1255426-1255208 [Citrobacter braakii]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCCTGCGACGTTGTCAGACAATTGACACACTTGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCACCGCCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT202335 WP_004150355.1 NZ_CP074541:c5430260-5430018 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT202335 NZ_CP099376:c1255828-1255454 [Citrobacter braakii]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCCACCAGCTTGCAGCTCAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGGTCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References